CC BY-NC-ND 4.0 · Laryngorhinootologie 2018; 97(S 02): S266
DOI: 10.1055/s-0038-1640635
Abstracts
Otologie: Otology

Stem cells contribute to the pathogenesis of middle ear cholesteatoma

H Sudhoff
1   Klinikum Bielefeld, Bielefeld, Deutschland
,
V Bortzmann
2   HNO-Klinik, Klinikum Bielefeld, Bielefeld
,
M Schürmann
2   HNO-Klinik, Klinikum Bielefeld, Bielefeld
,
J Nagel
2   HNO-Klinik, Klinikum Bielefeld, Bielefeld
,
B Kaltschmidt
3   Zellbiologie, Universität Bielefeld, Bielefeld
,
C Kaltschmidt
3   Zellbiologie, Universität Bielefeld, Bielefeld
,
I Todt
2   HNO-Klinik, Klinikum Bielefeld, Bielefeld
› Author Affiliations
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    Introduction:

    Cholesteatoma is a potentially life-threatening middle ear lesion due to the formation of an inflamed ectopic mass of keratinizing squamous epithelium. Surgical removal remains the only treatment option, emphasizing the need to gain a better understanding of this severe middle ear disease.

    Methods:

    Life sciences techniques, Gene expression Analysis.

    Results:

    We identified for the first time that stem cells residing in cholesteatoma tissue contribute to the pathogenesis of cholesteatoma. Cells expressing the “stemness” markers Nestin and S100B were detected in middle ear cholesteatoma and auditory canal skin. Isolated Nestin+/S100B+-cells showed the capability of self-renewal neurosphere formation and differentiation into mesodermal and ectodermal cell types including kerationcytes. Middle ear cholesteatoma-derived stem cells displayed an enhanced susceptibility to inflammatory stimulus compared to auditory canal skin stem cells, and this suggested a possible direct role in pathogenesis of cholesteatoma progression.

    Conclusions:

    Cholesteatoma-derived stem cells were able to differentiate into keratinocyte-like cells using factors mimicking the microenvironment of cholesteatoma. Our findings show a completely new perspective in the pathogenesis of cholesteatoma and may lead to new treatment strategies for this severe middle ear lesion.


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    No conflict of interest has been declared by the author(s).

    Professor Dr. med. D Holger Sudhoff
    Klinikum Bielefeld,
    Teutoburger Str. 50, 33604,
    Bielefeld,
    Deutschland   

    Publication History

    Publication Date:
    18 April 2018 (online)

    © 2018. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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