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DOI: 10.1055/s-0038-1647729
Intrinsic Activation of Prothrombin Potentiated by Celite and Reduced by Kaolin[*]
Publication History
Received
01 July 1974
Accepted
05 August 1974
Publication Date:
30 June 2018 (online)
Summary
Preparations of human prothrombin complex were absorbed with celite or kaolin and the 10% sodium chloride eluate of both absorbents was tested for its effectiveness to correct the prolonged partial thromboplastin time of celite-exhausted plasma. Both types of eluate were effective, indicating the presence of contact factors in the prothrombin preparations. Activation of the absorbed prothrombin complexes via the intrinsic pathway showed that while the kaolin absorbed preparations generated less thrombin than the parent solutions, celite absorbed complexes generated more thrombin than before they were absorbed. Both types of absorbed preparations generated the same amount of thrombin as before absorption when they were activated via the extrinsic pathway. When the absorption of the contact factors was performed by the addition of celite in the plasma before the purification of the prothrombin complex, the prothrombin preparations obtained generated less thrombin via the intrinsic pathway than the preparations which were obtained from plasma which had not been treated with celite.
* Contribution No. 293 from the American National Red Cross Blood Research Laboratory. Presented in part at the 58th Annual Meeting of the Federation of American Societies for Experimental Biology
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