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Thromb Haemost 1975; 33(02): 244-255
DOI: 10.1055/s-0038-1647878
Original Article
Schattauer GmbH

Molar Concentrations of Fibrinolytic Components, Especially Free Fibrinolysin, in Vivo

L. B Nanninga
1   Department of Physiology, University of Texas Medical Branch, Galveston, Texas 77550
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Publication History

Received 04 September 1973

Accepted 20 June 1974

Publication Date:
02 July 2018 (online)

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Summary

The levels of fibrinogen and of Profibrinolysin (plasminogen) in urokinase-treated plasma as a function of time of incubation were measured. The Profibrinolysin concentration was estimated through its complete conversion to fibrinolysin and the inhibition of the enzyme by crystalline soybean trypsin inhibitor. The dissociation constant of the FL-STX complex was determined to be 7 × 10–9 M. The average concentration of Profibrinolysin in normal human citrated plasma was found to be 8 × 10–7 M. From the decrease of fibrinogen with time in the urokinase-treated plasma, the free fibrinolysin was calculated. Free fibrinolysin in normal human blood in vivo was estimated from the half-life of fibrinogen and other data obtained in this study to be present at a concentration of 1.7 × 10–10 M. The plasmakinase activity in vivo, expressed as urokinase molarity, is also about 2 × 10–10 M.

 

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