Comparison of Two Types of Endothelial Cells in Long Term Culture^[*]

Summary

Bovine aortic endothelial cells have been serially subcultured and maintained for ten months (24 passages) without any apparent loss of endothelial-like properties. There appear to be two endothelial cell types; one type is mononucleated, 40—60 μ in diameter, and the other (giant) cell type, multinucleate and 300-600 μ in diameter. Both cell types were identified as endothelial cells by established criteria; 1. ultrastructurally, by the presence of Weibel-Palade bodies, microfilaments and pinocytotic vesicles and 2. immunologically, by the presence of factor VIII antigen and thrombosthenin.

^* Presented at the Vth Congress of the International Society on Thrombosis and Haemostasis, Paris, July, 1975.

^** Present address: The Department of Biological Sciences, Smith College, Northampton, Mass. 01060.

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