Effect of Alkaline and Acid Phosphatases on Clotting:

Summary

Alkaline phosphatase is found to enhance the activation of factor IX by SF. The correlation of the distribution of alkaline phosphatase in electrophoretic fractions with the clotting tests suggest that the “beta” fraction contains the responsible factor for the acceleratory effect of alkaline phosphatase on clotting.

Acid phosphatase, while not exerting a direct inhibitory effect on SF, does enhance the plasmatic anti-SF activity. The “beta” and “F-gamma” fractions seem to contain the responsible factor of acid phosphatase for the plasmatic anti-SF enhancement.

SF preparation has no acid or alkaline phosphatase activity.

A tentative schema is proposed to explain the effects of acid and alkaline phosphatase on clotting.

^* This study was aided by USPHS Research Grant No. HE01510-12.

• References

• 1 Ferguson J. H. Blood and body functions. F. A. Davis Co; Philadelphia: 1965
• 2 Macfarlane R. G, Biggs R, Ash B. J, Denson K. W. E. The interaction of factors VIII and IX. Brit. J. Haemat 10: 530 1964;
• 3 Davie E. W, Ratnoff O. D. Waterfall sequence for intrinsic blood clotting. Science 145: 1310 1964;
• 4 Ferguson J. H, Erickson B. N. The coagulant action of crystalline trypsin, cephalin, and lung extracts. Amer. J. Physiol 126: 661 1939;
• 5 Ferguson J. H. A new blood clotting theory. Science 97: 319 1943;
• 6 Seegers W. H. Prothrombin. Harvard Univ. Press; Cambridge, Mass: 1962
• 7 Ferguson J. H. Thromboplastic enzymes. Fed. Proc 23: 762 1964;
• 8 Alexander B. Some biochemical, physiochemical and immunochemical studies of prothrombin and proconvertin (factor VII): Their biopathologic significance. Proc. 4th Internat. Congr. Biochem. 1959
• 9 Kazal L. A. Interaction of phospholipids with lipoproteins, with serum and its protein, and with proteolytic and nonproteolytic enzymes in blood clotting. Trans. N.Y. Acad. Sci 27: 613 1965;
• 10 Iatridis P. G, Ferguson J. H. ATP-ase as an in vitro inhibitor of surface factor in the blood-clotting mechanism. Proc. Soc. exp. Biol. (N.Y.) 118: 647 1965;
• 11 Bettex-Galland M, Lüscher E. F. Thrombosthenin a contractile protein from thrombocytes. Its extraction from human blood platelets and some of its properties. Biochim. biophys. Acta (Amst.) 49: 536 1961;
• 12 Iatridis P. G, Ferguson J. H. The plasmatic atmosphere of blood platelets. Evidence that only fibrinogen, AcG, and activated Hageman factor are present on the surface of platelets. Thrombos. Diathes. haemorrh. (Stuttg.) 13: 114 1964;
• 13 Marcus A. J, Zucker M. B. The physiology of blood platelets: Recent Biochemical, Morphologic, and clinical research. Grune and Stratton; N.Y: 1965
• 14 Iatridis S. G, Ferguson J. H, Iatridis P. G, Mauldin R. Thrombogenic properties of surface factor: Evidence for an anti-surface factor activity in canine plasma. Thrombos. Diathes. haemorrh. (Stuttg.) 12: 35 1964;
• 15 Iatridis S. G, Ferguson J. H. Active Hageman factor: a plasma lysoldnase of the human fibrinolytic system. J. clin. Invest 41: 1277 1962;
• 16 Iatridis S. G, Ferguson J. H. A two stage assay method for Hageman factor activity. Thrombos. Diathes. haemorrh. (Stuttg.) 08: 46 1962;
• 17 Iatridis S. G, Iatridis P. G, Ferguson J. H. Roles of surface factor and a plasma inhibitory activity (Anti-SF) during experimental thrombosis in rabbits. Thrombos. Diathes. haemorrh. (Stuttg.) 12: 489 1964;
• 18 Langdeil R. D, Wagner R. H, Brinkhous K. M. Effect of antihemophilic factor on one- stage clotting tests. A presumptive test for hemophilia and a simple one-stage antihemophilic factor assay procedure. J. Lab. clin. Med 41: 637 1953;
• 19 Nye S. W, Graham J. B, Brinkhous K. M. The partial thromboplastic time as a screening test for the detection of latent bleeders. Amer. J. med. Sci 243: 55-279 1962;
• 20 Bell W. N, Alton H. G. A brain extract as substitute for platelet suspensions in the thromboplastin generation test. Nature (Lond.) 174: 880 1954;
• 21 Bessey O. H, Lowry O. H, Brock M. J. A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum. J. biol. Chem 164: 321 1946;
• 22 Andersch M. A, Szczypinski A. J. Use of p-nitrophenylphosphate as the substrate in determination of serum acid phosphatase. Amer. J. clin. Path 17: 571 1947;
• 23 Korner N. H. Distribution of alkaline phosphatase in serum protein fractions. J. clin. Path 15: 195 1962;
• 24 Nossel H. L. The contact phase of blood coagulation. F. A. Davis & Co; Philadelphia: 1964
• 25 Dubbs G. A, Vivonia G, Hilburn J. H. Subfractionation of human serum enzymes. Science 131: 1529 1960;
• 26 de Grouchy J. Répartition des activités estérasiques et phosphatasiques du sérum humain par rapport aux séro-proteines actuellement connues. Rev. franç. Étud. clin. biol 03: 881 1958;
• 27 Thomas D. P, Wessler S, Reimer S. M. The relation of factors XII, XI, and IX to hypercoagulable states. Thrombos. Diathes. haemorrh. (Stuttg.) 09: 90 1963;