Keywords
myringoplasty - tympanic membrane - platelet-rich fibrin - graft uptake
Introduction
Chronic suppurative otitis media is a chronic infection of the middle ear and the
mastoid cavity, which usually presents with purulent otorrhea for more than six weeks,
otalgia or hearing loss.
The global burden of chronic suppurative otitis media is estimated to be around 65
to 300 million patients, 60% of which experience significant hearing loss. The prevalence
of chronic suppurative otitis media is higher in developing countries[1] and ∼ 6% of the Indian population suffers from chronic ear disease.
Myringoplasty is a surgical procedure for repair of the tympanic membrane. The middle
ear cavity can be safeguarded from exposure to the external environment by repairing
the tympanic membrane, hence preventing further exposure to pathogens. The graft uptake
success rate may vary from 64–96%.[2] As per an audit published by the Royal College of Surgeons of England, in 1993,
and a prospective study done during the period of 2008 to 2012 the success rate of
graft uptake was found to be 80%.[3] A six-year prospective study conducted by Karela et al found the graft uptake success
rate to be as high as 91.6%.[4] After analyzing the database of 15 years and over 3,775 surgical procedures, the
Swedish National Registry estimated the graft uptake success rate to be around 88.5%.[5] A comparative study done by Murugendrappa et al found graft uptake to have a success
rate of 74% in myringoplasty done by conventional underlay technique.[6]
Platelet concentrates have been used extensively by maxillofacial surgeons, plastic
surgeons, and orthopedic surgeons to augment wound healing. The different platelet
concentrates that have been studied are platelet-rich plasma and platelet-rich fibrin.[7]
Platelet-rich fibrin is a second-generation platelet concentrate, which is rich in
platelets, cytokines, growth factors, and leukocytes that are trapped and released
over a period. It serves as a resorbable membrane that offers both mechanical and
inflammatory protection to the tympanic membrane. It accelerates cell proliferation
and matrix remodeling. Being an autologous biomaterial, it does not induce any undesirable
tissue reactions. Moreover, it is easy, quick and cheap to produce, and can easily
be manipulated during surgical procedures. The role of platelet-rich fibrin in the
repair of traumatic tympanic membrane perforation has been studied as well. As it
has strong elastic fibers rich with growth factors, it is considered an ideal patch
material for tympanic membrane perforation. Leukocytes embedded in the platelet-rich
fibrin scaffold also play a role in the release of growth factors, immune regulation,
antimicrobial response and matrix remodeling in wound healing.[8]
This study was done to analyze the efficacy of platelet-rich fibrin to aid graft uptake
success rate and the safety of its use in myringoplasty.
Objectives
To assess the safety and efficacy of autologous platelet-rich fibrin on graft uptake
in myringoplasty
Methodology
The present study was conducted between August 2015 and January 2017 in a tertiary
care center. The hypothesis was that the use of autologous platelet-rich fibrin is
safe in myringoplasty and improves the success rate of graft uptake in myringoplasty.
The sample size was calculated based on a study that reported the success rate of
graft uptake in myringoplasty as 64%.[9] The uptake rate was expected to improve a further 25% over routine myringoplasty
to 89%. The minimum required sample size was calculated using a 95% level of confidence
and 80% power, and it was found to be 43 in each group, study and control. (Using
the Open Epi statistical software).
A total of 86 patients, who were above the age of 18 and planned for myringoplasty,
were enrolled. Patients with comorbidities like diabetes and hypertension were excluded.
The study was designed to be a prospective, randomized controlled trial. Patients
were randomized into two groups by simple randomization. The random sequence was generated
using a random allocation software to allocate participants to either of the two groups.
The allocation was concealed using sequentially numbered sealed opaque envelopes.
The envelopes were then given to the scrub nurse and were opened at the time of recruitment
of the eligible participants.
In the study group, a sample of 10 ml of blood was collected from each patient in
a sterile container just before the surgery. The blood sample was centrifuged using
a table top centrifuge at 2,700 rpm for 12 minutes. Centrifugation resulted in the
formation of a fibrin clot (platelet-rich fibrin) in the middle of the tube (between
the RBC layer at the bottom and plasma at the top) ([Fig. 1]). The plasma layer is deficient in platelets. This middle layer was separated on
a laminar flow hood, under aseptic precautions ([Fig. 2]).[10] We were able to collect platelet-rich fibrin from all patients in the study group.
Around 1–2 mL of platelet-rich fibrin was collected from each patient.
Fig. 1 Post-centrifugation: layers of centrifuged blood.
Fig. 2 Platelet-rich fibrin clot.
Patients in both groups, control and study, underwent myringoplasty by postaural approach,
using the underlay technique and temporalis fascia graft. In the study group, after
placing the graft and repositioning the tympanomeatal flap, the platelet-rich fibrin
was placed as a plug over the sealed perforation ([Fig. 3]). In the control group, gel foam was kept over the sealed perforation, after repositing
the flap. Both groups of patients received antibiotics, as per the routine protocol
followed by the department.
Fig. 3 Platelet-rich fibrin clot kept as a plug over the sealed perforation.
Following the surgery, graft uptake was assessed by an otoscope at three months postsurgery
by a blinded observer ([Fig. 4]). The safety of platelet-rich fibrin was investigated with respect to immediate
postoperative infection in both groups, during routine follow-up. Patients presenting
with history of ear discharge, irrespective of bacteriological culture, were considered
to be postoperative infection in both groups. All patients who presented with discharge
were treated with antibiotics. One out of 43 patients in the control group was lost
to follow-up.
Fig. 4 Neotympanum, at three months of follow-up.
Statistical Analysis
The distribution of categorical variables, such as gender, postoperative infection
rate and graft uptake status, was expressed in terms of frequency and percentage.
Age was expressed as mean and standard deviation. The age analysis was performed with
independent Student t-test. The data of continuous variables, like duration of dry
ear and intraoperative perforation size, were converted to categorical variables,
which were expressed in terms of frequency and percentage. The comparison of these
categorical variables between the two groups was done by means of the Chi Square test.
All statistical analysis was performed with the SPSS Statistics package 17.0 (SPSS
Inc., Chicago, IL, USA). A p-value < 0.05 was considered significant.
Results
One patient was lost to follow-up during the study. Hence, the analysis was done as
per protocol, excluding the patient who was lost to follow-up. Seventy-one of the
patients recruited in the study were younger than 40 years of age. There was a predominance
of female patients among both groups. On assessing the duration of dry ear, it was
found that the majority of patients had more than six weeks of dry ear in both groups.
Among the groups, the majority of patients had tympanic membrane perforation involving
three or more quadrants. The ossicles were found to be intact in all patients, intraoperatively.
Nine patients were found to have middle ear inflammation ([Table 1]).
Table 1
Basic parameters in control and study group
|
Sl No
|
Parameters
|
PRF used (n = 43)
(Study)
|
PRF not used (n = 42)
(Control)
|
p-value
|
|
1
|
Age (years)*
|
31.91 ± 8.662
|
29.12 ± 9.572
|
0.163
|
|
2
|
Gender (male/female)
|
19/24
|
20/22
|
0.751
|
|
3
|
Ear dry duration in weeks
(0–2 / 2–6 / > 6)
|
3/12/28
|
1/10/31
|
0.516
|
|
4
|
Ear side
(right/left)
|
12/31
|
17/25
|
0.222
|
|
5
|
Intra operative middle ear inflammation
|
5/38
|
4/38
|
0.753
|
|
6
|
Number of tympanic membrane quadrants involved by perforation
1/2/3/4
|
1/14/10/18
|
2/6/6/28
|
0.082
|
Abbreviation: PRF, platelet-rich fibrin.
*Age is expressed in mean years ± standard deviation.
A total of 4.7% of the patients in whom the platelet-rich fibrin was used was found
to have an infection, whereas in the control group, 19% of the patients had postoperative
infection. ([Table 2]).
Table 2
Comparison of postoperative infection among study and control group
|
Sl No
|
Platelet-rich fibrin
|
Post-operative infection present
|
No post-operative infection
|
Total
|
|
1
|
Used
|
2 (4.7%)
|
41 (95.3%)
|
43
|
|
2
|
Not used
|
8 (19%)
|
34 (81%)
|
42
|
p-value= 0.039.
At three months of follow-up, 97.7% of patients had successful graft uptake. Meanwhile,
81% of control cases had successful graft uptake. ([Table 3]).
Table 3
Comparison of postoperative graft uptake among study and control group
|
Sl No
|
Platelet-rich fibrin
|
Postoperative graft uptake present
|
No postoperative graft uptake
|
Total
|
|
1
|
Used
|
42 (97.7%)
|
1 (2.3%)
|
43
|
|
2
|
Not used
|
34 (81%)
|
8 (19%)
|
42
|
p-value =0.012.
Of the nine patients who had had intraoperative middle ear inflammation, all of them
had successful graft uptake, and none presented with postoperative infection. Four
of these patients belonged to the study group, and five to the control group.
Most patients had perforation involving three or more quadrants. Among the 34 patients
in the control group, 27 (79%) had successful graft uptake. However, all patients
in the study group had successful graft uptake ([Table 4]).
Table 4
Comparison of graft uptake among study and control group, who had intraoperatively
perforation involving three or more quadrants
|
Sl No
|
Platelet-rich fibrin
|
Postoperative graft uptake present
|
No postoperative graft uptake
|
Total
|
|
1
|
Used
|
28 (100%)
|
0
|
28
|
|
2
|
Not used
|
27 (79%)
|
7 (21%)
|
34
|
p-value = 0.011 by Fischer Exact t-test.
Seven out of 34 patients (21%) in the control group had postoperative infection while
only 1 out of 28 patients in the study group had postoperative infection (3.6%). All
patients had successful graft uptake at the end of 3 months ([Table 5]).
Table 5
Comparison of postoperative infection among study and control group, in patients who
had intra-operatively perforation involving more than three quadrants
|
Sl No
|
Platelet-rich fibrin
|
Postoperative infection present
|
No postoperative infection
|
Total
|
|
1
|
Used
|
1 (3.6%)
|
27 (96.4%)
|
28
|
|
2
|
Not used
|
7 (21%)
|
27 (79%)
|
34
|
p-value = 0.05 by Fischer Exact t-test.
Among the 4 patients (1 = control group; 3 = study group) who had a history of dry
ear for less than 2 weeks, only the patient in the control group had any infection.
Patients in the study group had successful graft uptake and had no postoperative infection.
Among the 59 patients (31 in the control group; 28 in the study group) who had a history
of dry ear of more than 6 weeks, the graft uptake success rate was found to be 87.1%
in the control group, as opposed to 100% in the study group ([Table 6]). None in the study group had postoperative infection; while, 12.9% of controls
had postoperative infection ([Table 7]).
Table 6
Comparison of graft uptake among study and control group in patients with history
of ear dry duration more than six weeks from the time of surgery
|
Sl No
|
Platelet-rich fibrin
|
Post-operative graft uptake present
|
No post-operative graft uptake
|
Total
|
|
1
|
Used
|
28
|
0
|
28
|
|
2
|
Not used
|
27 (87.1%)
|
4 (12.9%)
|
31
|
p-value = 0.029 (Fischer Exact t-test).
Table 7
Comparison of post-operative infection among study and control group in patients with
history of ear dry duration more than six weeks from the time of surgery
|
Sl No
|
Platelet-rich fibrin
|
Post-operative infection present
|
No post-operative infection
|
Total
|
|
1
|
Used
|
0
|
28 (100%)
|
28
|
|
2
|
Not used
|
4 (12.9%)
|
27 (87.1%)
|
31
|
p-value = 0.029 (Fischer Exact t-test).
Among the 25 patients, (15 in the study group; 10 in the control group) with history
of dry ear of less than 6 weeks, the graft uptake success rate was found to be 91.6%
in the study group, as compared with 66.6% in the control group. The postoperative
infection rate was found to be in 8.4% of the patients in the study group and 33.6%
in the control group. (p > 0.05; not statistically significant)
Discussion
The analysis of age distribution revealed no statistically significant difference
between the study and control groups. The majority of the patients recruited were
young, with 55.7% of the patients in the 18–30 years age group. The majority of the
patients recruited for this study were female (54.1%), which may be considered an
indicator of increased treatment seeking behavior amidst young and female patients.
A total of 95.3% of patients gave history of dry ear with duration of more than two
weeks. There were four patients who had had a dry ear for less than 2 weeks. On analyzing
the graft uptake success rates, even in patients with dry ear duration of less than
2 weeks, the success rates among the study population was 100%. Furthermore, there
was no postoperative infection. Only one patient in the control group had postoperative
infection and graft uptake failure. However, the numbers in either group are too few
to comment
On detailed perusal of the available data, it becomes evident that the graft uptake
rate was better in cases in which platelet-rich fibrin was used. The graft uptake
rate was found to be 97.7% in the study group as opposed to 81% in the control group.
In other words, there was a difference of 16.7% between the two groups.
Previous studies had shown a success rate of myringoplasty ranging from 64–96%. The
audit published by the Royal College of Surgeons of England in, 1993, reported a success
rate of 65%, which improved to 80% in 1997.[3] A prospective study done by Kamath et al over the period of 2008 to 2012 had found
a graft uptake success rate of 80% among 60 cases.[11] From this, we can understand that the graft uptake success rate in our study was
comparable and almost similar to other studies.
Among all platelet concentrates, platelet-rich plasma has been extensively studied.
In otology, it is used for traumatic tympanic membrane perforation, type 1 tympanoplasty,
for facial nerve regeneration, etc. The biostimulation effect of platelet-rich plasma
on type 1 tympanoplasty studied by Navarrete Álvaro et al showed that platelet-rich
plasma stimulates fibroblast and thus, aids in the closure of tympanic membrane perforations.[12]
A study done by Sankaranarayanan et al on platelet-rich plasma-assisted tympanoplasty
found that closure rates were better in patients in whom platelet-rich plasma was
used, as compared with a control group. They assessed 25 patients in each group and
found a closure rate of 92% in the study group and 70% in the control group.[13]
Topical application of autologous platelet-rich plasma was analyzed by El-Anwar et
al. They concluded that topical autologous platelet-rich plasma is safe and enhances
the healing of chronic tympanic membrane perforation and avoid infections.[14] In another study by El Anwar et al, patients with posttraumatic tympanic membrane,
they freshened the margins and applied autologous platelet-rich plasma in an hourglass
manner. The study was done on an office basis. They found that in 84% of the cases
the tympanic membrane had healed completely. They concluded in their study that office-based
platelet-rich plasma myringoplasty is an effective and safe minimally invasive procedure.[15]
A study was conducted by Habesoglu et al in 2011 among 32 patients with acute tympanic
membrane perforation. He concluded that the use of platelet-rich fibrin accelerated
the tympanic membrane closure.[16] An animal study conducted by Ensari et al assessed the healing rate of traumatic
perforation in rats. He found that the mean healing time of the perforation was decreased
in the study group, in which the perforation was closed by platelet-rich fibrin.[17]
In this same study, we find that the graft uptake success rate was increased by 16.7%
in patients in whom platelet-rich fibrin assisted tympanoplasty was done. When we
separately analyzed patients with perforations involving three or more quadrants,
the graft uptake rate in the study group was found to be 100%, in comparison to 79%
in the control group.
It is also observed that the postoperative infection rates were fewer in the study
group as compared with the control group. In fact, there was a difference of 14.3%
concerning postoperative infection rate. Furthermore, on assessing patients with tympanic
membrane perforation involving three or more perforations, the postoperative infection
rates were found to be lower in the study group.
In patients with middle ear inflammation, the graft uptake rate was found to be 100%
in both groups. No patient presented with postoperative infection in either group.
It has also been seen that in larger perforations, the use of platelet-rich fibrin
increased the graft uptake chances and reduced the intraoperative infection rate.
The same results were observed regarding dry ear duration. The majority of patients
belonged to the group with history of dry ear for more than 6 weeks.
Garin et al found that platelet-rich fibrin can be used safely for middle ear packing
without any adverse effects, such as those observed with collagen derived from porcine
skin. He concluded that it could be used as a substitute to collagen.[18]
In a review article by Sarkar,[2] it was found that filling the cavity with platelet-rich fibrin following third molar
extraction led to a 10-fold reduction in the risk of osteomyelitis.[2]
We have seen that platelet-rich fibrin improves the graft uptake rate. This also serves
as evidence pertaining to the safety aspect of platelet-rich fibrin, as it prevents
the exposure of the middle ear to the environment. Moreover, on analyzing the results,
we see that there is a decreased incidence of postoperative infection in patients
in whom platelet-rich fibrin was used as compared with the control group. Being autologous,
platelet-rich fibrin is safe, cheap and easily available. It is not expected to cause
any allergic/tissue reactions, which can occur with allogenous platelet-rich fibrin.
Based on the above studies, it is hypothesized that the antimicrobial effect would
have been the cause of the reduced infection rate in the study group.
This study was a randomized control trial. There have been very few studies that have
analyzed the safety and efficacy of platelet-rich fibrin in otology to date. The sample
size was achieved. As an autologous material was used, there were no undesirable reactions.
However, long-term efficacy and safety have not been addressed in this study.
Conclusion
As there was no significant number of postoperative infections in platelet-rich fibrin
aided myringoplasty, it is considered safe for use in myringoplasty. The procedure
of production of platelet-rich fibrin is simple; it is cheap, safe and easily available.
The chances of graft uptake are better when myringoplasty is aided with platelet-rich
fibrin, especially in case of larger tympanic membrane perforation. The graft uptake
success rate was observed to be better in patients belonging to different groups of
dry ear duration. The reduced postoperative infection rate in the platelet-rich fibrin-aided
myringoplasty group may suggest the antimicrobial effect of platelet-rich fibrin.
However, further studies are required to support this fact.
Hence, we conclude that platelet-rich fibrin is safe and efficacious in myringoplasty.
