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Thromb Haemost 1968; 19(03/04): 368-382
DOI: 10.1055/s-0038-1651215
Originalarbeiten – Original Articles – Travaux Originaux
Schattauer GmbH

Kinetic Aspects of the Interaction of Blood Clotting Enzymes

V. The Reaction Mechanism of the Extrinsic Clotting System as Revealed by the Kinetics of One-Stage Estimations of Coagulation Enzymes
H. C Hemker
1   Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Depts. of Haematology and Cardiology, Clinic for Internal Medicine, University Hospital, Leiden, The Netherlands
,
A. D Muller
1   Laboratories for Coagulation Biochemistry and Cardiovascular Biochemical Research, Depts. of Haematology and Cardiology, Clinic for Internal Medicine, University Hospital, Leiden, The Netherlands
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Publication Date:
27 June 2018 (online)

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Summary

Observations of the clotting time in one-stage tests in the extrinsic system show, after suitable correction, that there is a rectilinear relationship between the clotting time and the inverse of the concentration of the rate-limiting factor. This cannot be explained by the “cascade” or “waterfall” mechanism proposed as a scheme for the interaction of the blood-clotting factors concerned. This relationship can, however, be explained by a slightly different mechanism.

 

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