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Thromb Haemost 1975; 34(01): 003-018
DOI: 10.1055/s-0038-1651441
International Committee Business
Schattauer GmbH

Standard for Human Thrombin

I Robertson
1   National Institute for Biological Standards and Control, Hampstead, London NW3 6RB
,
P. J Gaffney
1   National Institute for Biological Standards and Control, Hampstead, London NW3 6RB
,
D. R Bangham
1   National Institute for Biological Standards and Control, Hampstead, London NW3 6RB
› Author Affiliations
Further Information

Publication History

Publication Date:
02 July 2018 (online)

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Summary

Ten laboratories took part in an international study of the proposed standard for human thrombin. A freeze-dried preparation (coded 70/157) was shown, using clotting time tests, to be suitable to serve as a standard for the assay of human and bovine thrombin preparations. The study shows that assay variation within laboratories was much less than between laboratories. The preparation was stable, having a negligible estimated loss of potency after ten years under the recommended storage conditions (—20° C in the dark).

Each ampoule of the standard was assigned a potency of 100 units of thrombin activity, and one unit was defined as the activity contained in 0.0853 mg of the freeze-dried preparation.

It was agreed by the participants in the study and by the International Committee on Thrombosis and Haemostasis (Vienna, 1973) that the thrombin preparation coded 70/157 is suitable to serve as an international standard for human thrombin.

 

  • References

  • 1 Blombäck B, and Blombäck M. 1956; Purification of human and bovine fibrinogen. Arkiv. Kemi 10: 415.
    PubMedGoogle Scholar
  • 2 Blombäck B. 1969 Selectional trends in the structure of fibrinogen of different species. Symposium of the Zoological Society (London), 27, 167; Academic Press (London).
    PubMedGoogle Scholar
  • 3 Campbell P. J. 1974; a International biological standards and reference preparations I. Preparation and presentation of materials to serve as standards and reference preparations. Journal of Biological Standardization 2: 249.
    CrossrefPubMedGoogle Scholar
  • 4 Campbell P. J. 1974; b International biological standards and reference preparations II. Procedures used for the production of biological standards and reference preparations. Journal of Biological Standardization 2: 259.
    CrossrefPubMedGoogle Scholar
  • 5 Davis B. J. 1964; Disc electrophoresis II. Method and application to human serum protein. Annals of the New York Academy of Science 21: 404.
    PubMedGoogle Scholar
  • 6 Jerne N. K, and Perry W. L. M. 1956; The stability of biological standards. Bulletin of the World Health Organization 14: 167.
    PubMedGoogle Scholar
  • 7 Johnson A. J, Kline D. L, and Alkjaersig N. 1969; Assay methods and standard preparations for plasmin, plasminogen and urokinase in purified systems, 1967-1968. Thrombosis et Diathesis Haemorrhagica 21: 259.
    PubMedGoogle Scholar
  • 8 Jorpes E, Vrethammar T, Ottman B, and Blombäck B. 1958; On the assay of thrombin preparations. Journal of Pharmacy and Pharmacology 10: 561.
    CrossrefPubMedGoogle Scholar
  • 9 Miller K. D, and Copeland W. H. 1962; Isolation of human thrombin. Proceedings of the Society of Experimental Biology and Medicine 111: 512.
    PubMedGoogle Scholar
  • 10 Teger-Nilsson A. C, and Blombäck B. 1974; Bate of thrombin-fibrinogen reaction in some mammalian species. Thrombosis Research 5: 223.
    CrossrefPubMedGoogle Scholar
  • 11 Weber K, and Osborn M. 1969; The reliability of molecular weight determinations by dodecyl-sulphate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244: 4406.
    PubMedGoogle Scholar