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Thromb Haemost 1975; 34(03): 780-794
DOI: 10.1055/s-0038-1651471
Original Article
Schattauer GmbH

Colchicine Uptake and Binding by Human Platelets

Dianne M Kenney
1   Center for Blood Research, Department of Medicine, Harvard Medical School and New England Deaconess Hospital, Boston, Massachusetts U.S.A.
,
Francis C Chao
1   Center for Blood Research, Department of Medicine, Harvard Medical School and New England Deaconess Hospital, Boston, Massachusetts U.S.A.
,
James L Tullis
1   Center for Blood Research, Department of Medicine, Harvard Medical School and New England Deaconess Hospital, Boston, Massachusetts U.S.A.
,
Gail S Conneely
1   Center for Blood Research, Department of Medicine, Harvard Medical School and New England Deaconess Hospital, Boston, Massachusetts U.S.A.
› Author Affiliations
Further Information

Publication History

Received 23 March 1975

Accepted 26 July 1975

Publication Date:
02 July 2018 (online)

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Summary

The uptake and binding of antimitotic alkaloid colchicine has been demonstrated in washed preparations of human platelets. A silicone oil technique was adapted so that both uptake and binding of 14C-colchicine were examined in the same platelet preparations. The time dependence and amount of colchicine taken up and bound by different platelet preparations during a 90 to 120 min incubation period were highly reproducible. Both colchicine uptake and binding by intact platelets, and colchicine binding by preparations of lysed platelets were specific and temperature dependent. Colchicine uptake was slowly reversible. Magnesium and GTP enhanced colchicine binding by lysed platelet preparations but calcium decreased binding.

Exposure of platelets to either cold (4° C) or to thrombin, which disrupt platelet microtubules, produced significant increases in colchicine uptake and binding. The thrombin effect was maximal at 37° C and resulted in a greater increase in uptake and binding than that produced by either cold treatment alone or, by cold treatment followed by incubation with thrombin at 37° C. The amount of increase in uptake and binding produced by thrombin was independent of both thrombin (1–5 Units/109 platelets) and colchicine concentrations (1–50 × 10−6M).It is postulated that thrombin may initiate the formation, or make available, colchicine binding sites (microtubule subunits) within platelets.

 

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