Blood Coagulation

Summary

Preparations of platelet factor 3 accelerated the conversion of purified autoprothrombin III (F-X) to autoprothrombin C (F-X?). The reaction was slow and the yields were low. Purified Hageman factor or purified autoprothrombin II (F-IX?) functioned as platelet cofactors. Each increased the yield of autoprothrombin C from purified autoprothrombin III above that obtained with platelet factor 3 alone. Hageman protein thus has a role in the direct acceleration of the first basic reaction in blood coagulation. Purified prothrombin was made refractory to the two-stage analytical reagents with Hageman factor.

^* This work was supported by a research grant HE-03424-13 from the National Heart Institute, National Institutes of Health, U.S. Public Health Service. We wish to thank Eberhard F. Mammen for helpful suggestions and discussions.

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