Platelet Aggregation by a Specific Human Immunoglobulin Preparation

Summary

A patient with severe chronic thrombocytopenic purpura is described, whose serum, after repeated transfusions of platelet-rich plasma, of her ABO blood group, was found to contain immunoglobulin G, which produced clumping in platelet-rich plasma from 15 volunteers out of 20. Direct and indirect evidence was obtained that clumping was due to release of endogenous platelet-ADP. In addition, a direct effect of the immunoglobulin preparation on the platelet membrane could be demonstrated.

• References

• Ardlie N. G. ADP-induced platelet aggregation without a plasma cofactor. Fed. Proc 27: 321 1968;
• Born G. V. R. Aggregation of blood-platelets by adenosine-diphosphate and its reversal. Nature (Lond) 194: 927-929 1962;
• Born G. V. R, Gross M. J. Effects of inorganic ions and of plasma proteins on the aggregation of blood-platelets by adenosine-diphosphate. J. Physiol 170: 397-414 1964;
• Caen J, Legrand Y. L’analyse des fonctions plaquettaires. Adhésion et agrégation des plaquettes au collagène purifié. Rev. franç. Etud. clin. Biol 13: 1028-1030 1968;
• Coombs R. R. A, Lachmann P. J. Immunological reactions at the cell surface. Brit. med. Bull 24: 113-120 1968;
• Dausset J, Colin M, Colombani J. Immune platelet iso-antibodies. Vox Sang 05: 4-31 1960;
• Hardisty R. M, Hutton R. A. Platelet aggregation and availability of platelet factor 3. Brit. J. Haemat 12: 764-776 1966;
• Haslam R. J. Role of adenosine-phosphate in the aggregation of human blood-platelets by thrombin and by fatty acids. Nature (Lond) 202: 765-768 1964;
• Mueller-Eckhardt Ch, Lüscher E. F. Immune reactions of human blood-platelets. I. A comparative study on the effects on platelets of heterologous antiplatelet antiserum, antigen-antibody complexes, aggregated gammaglobulin and thrombin. Thrombos. Diathes. haemorrh. (Stuttg) 20: 155-167 1968;
• Murer E, Hellem A, Rozenberg M. C. Energy metabolism and platelet function. Scand. J. clin. Lab. Invest 19: 280-282 1967;
• O’Brien J. R. Effects of salicylates on human platelets. Lancet I 779-783 1968;
• O’Brien J. R, Heywood J. B, Heady J. A. The quantitation of platelet aggregation induced by four compounds: a study in relation to myocardial infarction. Thrombos. Diathes. haemorrh. (Stuttg) 16: 752-767 1966;
• O’Brien J. R, Shoobridge S. M, Finch W. V. Comparison of the effect of heparin and citrate on platelet aggregation. J. clin. Path 22: 28-31 1969;
• Roskam J. Proposals for certain definitions in haemostasis and thrombosis. Transactions of the Conference held under the Auspices of the International Committee of Haemostasis and Thrombosis. St. Moritz; Switzerland: September 1965: 317-319 F. K. Schattauer, Stuttgart 1965
• Scheidegger J. J. Une microméthode de l’immunoélectrophorèse. Int. Arch. Allergy 07: 103-110 1955;
• Spaet T. H, Cintron J. Studies on platelet factor 3 availability. Brit. J. Haemat 27: 269-275 1965;
• Vaerman J. P, Heremans J. F, Vaerman C. Studies of the immune globulins of human serum. J. Immunol 91: 7-10 1963;
• Van de Wiel J. W. M, van de Wiel-Dorfmeyer H, van Loghem J. J. Studies on platelet antibodies in man. Vox. Sang 06: 641-668 1961;