Synthesis of the Enantiomers of Thioridazine

Abstract

Thioridazine, a well-known antipsychotic drug, has shown promising effects on several bacterial strains (including Mycobacterium tuberculosis and methicillin-resistant Staphylococcus aureus). Suppressive effects towards selected cancer cell-lines have also been reported. However, due to adverse effects, the compound is no longer in use for the primary indication. More recent research has demonstrated that these side effects are limited to one of the two enantiomers, (+)-thioridazine. The question arises to whether the beneficial effects of thioridazine are limited to one enantiomer, or if (–)-thioridazine can prove itself to be useful in its pure enantiomeric state. The published procedures on the synthesis of the optically pure enantiomers of thioridazine were found to be unsatisfactory, either due to low optical purity, high cost, or problems scaling up. Herein, we have used an auxiliary-based strategy for the total synthesis of both enantiomers in high optical purity and good overall yield. The strategy can easily be scaled up. Both enantiomers were tested against several bacteria. Comparison of the racemic mixture, (–)-thioridazine and its (+)-antipode revealed that they have the same antimicrobial effects. Thus, the non-toxic enantiomer, (–)-thioridazine, can prove useful in this role and should be investigated further.

Key words

thioridazine - antibacterial - enantiomers - total synthesis - sulfinyl aldimines

Phenothiazine drugs are well-known antipsychotic drugs. Members of this drug class include thioridazine (1), fluphenazine (2) and chlorpromazine (3), the first antipsychotic drug on the market, and methylene blue (4) (Figure [1]). Thioridazine (1) was introduced as a treatment for schizophrenia and psychosis, and soon became widely used.[1] Compared to chlorpromazine, thioridazine results in less pronounced extrapyramidal side effects.[2] [3]

Figure 1 Structures of four well-known phenothiazines

A common side effect of antipsychotic drugs is a prolonged QT interval, i.e., the time from when the cardiac ventricles start to contract until they are finished relaxing, and this was soon reported to be the case for thioridazine.[4] Therapeutic doses can cause cardiac repolarization abnormalities and prolongation of QT in a dose-dependent manner.[5] [6] Cases of the potentially fatal ventricular arrhythmia, Torsades de Pointes, have been reported. To date, 41 sudden deaths in patients have been associated with treatment with thioridazine.[5] ^, [7–12] Around the turn of the millennium, the risks for these severe side effects were found to be higher than for other antipsychotic drugs.[13] Accordingly, branded versions of thioridazine were withdrawn in 2005.[14]

In addition to antipsychotic properties, several members of the phenothiazine class have significant antimicrobial activity against a wide range of bacteria.[14] However, thioridazine has not been used clinically as an antibacterial agent. Nevertheless, thioridazine has attracted renewed interest­ in recent years due to its activity against multi-drug-resistant and extensively drug-resistant tuberculosis.[15] Particularly interesting are reports of increased sensitivity of drug-resistant Mycobacterium tuberculosis and methicillin-resistant Staphylococcus aureus (MRSA) to thioridazine.[7] [15] [16] The suggested mechanism of action is inhibition of bacterial efflux pumps.[17]

Moreover, it has been demonstrated that thioridazine is concentrated in macrophages. Infections in macrophage-rich tissue (e.g., lung, kidney, spleen and liver) include tuberculosis. Consequently, lower doses are expected to be needed for the treatment of such infections, compared to treatment of psychosis.[18] [19] [20] [21]

Furthermore, thioridazine has the ability to suppress the growth of several types of cancer cells.[22] Recently, studies revealed thioridazine to target cancer stem cells selectively.[23] [24] [25] It has also been demonstrated that thioridazine makes multidrug-resistant cancer cells vulnerable to cytotoxic agents to which they were originally resistant.[26]

The majority of the reports on thioridazine are concerned with the biology of the commercially available thioridazine, which is the hydrochloride of the racemate. During the last 30 years, several groups have investigated the properties of the separate enantiomers, rather than the racemate. Experiments in rat brains revealed that the (+)-enantiomer has approximately 2.7 times greater affinity than the (–)-enantiomer for the D₂ dopamine receptor, the inhibition of which results in the antipsychotic effect of thioridazine. The (+)-enantiomer also has 4.5 times higher affinity for the norepinephrine α1 receptor. In contrast, (–)-thioridazine has 10 times higher affinity for the D₁ dopamine receptor.[27] [28]

The (–)-enantiomer causes significantly less ventricular action potential prolongation of the rabbit papillary muscle compared to (+)-thioridazine.[29] With its reduced antipsychotic effects and reduced prolongation of APD, (–)-thioridazine warrants further biological investigation.

There are only a couple of published procedures for the preparation of enantiomers of thioridazine.[30] [31] [32] Bourquin et al. reported the first synthesis of 1, starting from racemic piperidine alcohol 5 (Scheme [1]). Enantiomers of 1 were separated via chiral resolution employing di-p-tolyl-l-tartaric acid as a chiral derivatizing agent, making this a rather inefficient synthesis of the desired enantiomer.[30]

Scheme 1 Synthesis by Bourquin et al.[30] Reagents and conditions: (i) thionyl chloride, CHCl₃, 70 °C, 90% yield; (ii) NaNH₂, phenothiazine 7, PhCH₃, xylene, 180 °C, 60% yield.

An alternative to this approach starts with optically pure piperidine-2-carboxylic acid;[33] the synthesis is shown in Scheme [2]. Piperidine-2-carboxylic acid is treated under Eschweiler–Clarke conditions followed by esterification. The ester is reduced using LiAlH₄ and transformed into the corresponding nitrile. The nitrile is hydrolyzed with hydrochloric acid in methanol and the resulting methyl ester is reduced to the alcohol and treated with thionyl chloride. The specific rotation of the resulting chloride (–)-6 was compared with the patent literature in order to define the absolute stereochemistry of (+)-1 and (–)-1 as R and S, respectively.[33] It should also be noted that (–)-5 can be made through chiral resolution, but with tedious and low-yielding nine recrystallizations to obtain sufficient optical purity. Most of the eight steps are high yielding, but the overall yield is 8% and the starting material, piperidine-2-carboxylic acid, is quite expensive and constitutes a limitation for scale up.

Scheme 2 Synthesis of the thioridazine developed by Patrick and Singletary­.[33] Reagents and conditions: (i) HCO₂H, CH₂O, 16 h, reflux, 76%; (ii) AcCl, 3 M HCl, EtOH, 18 h, reflux, 89%; (iii) LiAlH₄, Et₂O, reflux, 6 h, 81%; (iv) KCN, 18-crown-6-ether, MeCN, Bu₃P, 36 h, reflux, 80%; (v) HCl in MeOH, 2 °C, 67%; (vi) LiAlH₄, Et₂O, reflux, 6 h, 90%; (vii) CHCl₃, HCl (gas), Cl₂SO, 5 h, 0 °C, 51%; (viii) NaOH, 7, PhCH₃, reflux, 3 h, 60% (for final reaction refer to Bourquin et al.[30]).

Another alternative is the procedure reported by Choi et al., in which the menthyl carbamate of 1 is prepared and the diastereomers are separated by flash chromatography. However, the carbamate is easily hydrolyzed, which makes the separation step hard to scale up. LiAlH₄ reduction of these carbamates gives the enantiomers of 1.[31] This procedure gives good enantiomeric excess, but the overall yield is poor. In addition, the production of larger quantities is laborious, due to the bottleneck of the diastereomer separation step.

Mohammad et al. reported the total syntheses of racemic thioridazine and isotope-labeled analogues.[34] [35] The strategy is elegant, but not suited for asymmetric synthesis. Accordingly, we decided to develop our own total synthesis that can be scaled up to produce larger volumes of both enantiomers.

Our key step involves the preparation of enantiopure alcohol 5. The retrosynthesis is depicted in Scheme [3]. The piperidine-2-carboxylic acid approach is opportune, as the stereogenic center is present in the starting material. However­, previously we have observed the lability towards racemization of α-amino acids during elongation.[36] The allyl moiety is easy to handle, and can be oxidized to an aldehyde in a straightforward manner. For asymmetric synthesis of amines, chiral auxiliaries, for example, the use of chiral sulfonamides, are excellent choices. It is also known that sulfonamides can be selectively alkylated in an aqueous environment.[37] After hydrolysis, the sulfonamides can be recovered and reused.[38]

Scheme 3 Retrosynthesis

First, we aimed for the S-enantiomer of 1. Condensation of 5-chloropentanal 9 with (S)-tert-butanesulfinamide, gave the corresponding N-tert-butanesulfinyl aldimine 10 (Scheme [4]). Indium-mediated allylation of (R)-10 in aqueous media, gave (R,S)-11 and (R,R)-11 in close to 10:1 dia­stereomeric ratio and quantitative yield. The two diastereomers were separated by flash chromatography to give single diastereomers, and the major product, (R,S)-11, was used in the next reaction.[39]

Scheme 4 Synthesis of the S-enantiomer of thioridazine. Reagents and conditions: (i) (S)-tert-butanesulfinamide, CuSO₄, CH₂Cl₂, r.t., overnight, 91%; (ii) allyl bromide, NaBr, In, H₂O, r.t., overnight, quant; (iii) HCl, 1,4-dioxane, 1 h, r.t.; (iv) K₂CO₃, DMF, 15 min, 120 °C microwave heating; (v) K₂CO₃, Boc₂O, THF, 0 °C, overnight; 64% over three steps; (vi) OsO₄ (2.5wt% in tert-butanol), 1,4-dioxane, 2,6-lutidine, 5 h, r.t. 82%; (vii) LiAlH₄ (2.0 M in THF), 50 °C, 18 h, 88%; (viii) SOCl₂, CHCl₃, reflux, 3 h, quant; (ix) NaOH, 7, PhCH₃, reflux, 3 h, 81%.

Acid-mediated hydrolysis of the tert-butanesulfonamide, with a subsequent intramolecular alkylation, best achieved using microwave heating, yielded the piperidine motif. Next, the amine was protected as the tert-butyl-carbamate­ (S)-12, to avoid possible formation of the N-oxide, during the Lemieux–Johnson oxidation. The oxidation worked well, yielding aldehyde (S)-13. Focusing on the active nitrogen in the phenothiazine ring, reductive amination was envisaged as an alternative. However, this approach proved unsuccessful as we only recovered starting material.

The carbamate functionality was hydrolyzed, and the resulting piperidine was methylated under Eschweiler–Clarke conditions. Focusing on the active nitrogen in the phenothiazine ring, reductive amination was considered as an alternative. However, this also proved unsuccessful.

Finally, LiAlH₄ reduction of the carbamate (S)-13 gave amino alcohol (S)-5. All data are in accordance with published data. Amino alcohol (S)-5 was transformed into the corresponding chloride (S)-6 and coupled with phenothiazine 7, to give the (S)-enantiomer of thioridazine.[30]

Corresponding reactions starting from (R)-tert-butanesulfinamide gave the (R)-enantiomer of thioridazine.

The optical purity of the target molecule was checked by measurement of the specific rotation and by chiral HPLC. The specific rotations were found to be the same as reported for both enantiomers.[30] For the chiral HPLC analysis, the method reported by Schaluder et al. was used.[40] As reported, full separation of the two enantiomers on chiral HPLC was not achieved. Different chiral columns, different eluent mixtures and different base additives were tested, but only partial separation was obtained. However, for the scalemic samples only single peaks were observed. In combination with the specific rotation values, that were the same as reported in the literature, we therefore conclude that our samples were of high enantiopurity.

Minimum inhibitory concentrations (MIC) against different strains of seven bacteria were measured for both enantiomers and the racemate (Table [1]). Thioridazine racemate and the pure enantiomers were found to be relatively active against the Gram-positive bacteria Mycobacterium samegmatis, Staphylococcus aureus MRSA, Enterococcus faecalis, and Enterococcus faecium, but not effective against the Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. The compounds are also slightly active against nosocomial strains of Acinetobacter baumannii, which are difficult to treat in hospitals due to their resistance to most antibiotics. The activities of the enantiomers do not differ significantly from those of the racemic mixture.

Table 1 Minimum Inhibitory Concentrations (MIC) of Thioridazine (μg/mL) toward a Selected Set of Important Pathogens

Bacteriaa	Compounda
	rac	+	–
M. smegmatis (n=2)	10	10	10
E. faecalis (n=4)b	20	20	20
MRSA (n=4)	20–40	20–40	20–40
E. faecium (n=4)	20	20	10–20
A. baumanii (n=4)c	80–160	80–160	40–160
E. coli (n=3)	>1280	>1280	>1280
P. aeruginosa (n=2)	>1280	>1280	>1280

^a The number of tested strains of each species given as n.

^b Including one nosocomial VRE.

^c Nosocomial strains.

In conclusion, both enantiomers of thioridazine have been prepared in high optical purity and good overall yield.[41] The route via Lemieux–Johnson oxidation of sulfonamide 11 followed by global reduction to give compound 5 proves to be a good, and cheaper alternative to both enantiomers of thioridazine 1.

Antimicrobial effects of the two enantiomers are comparable to the effects of the racemate. Of special interest is Mycobacterium smegmatis, as its genus includes harmful pathogens, including Mycobacterium tuberculosis and Mycobacterium leprae. Similar findings were also reported by Poulsen et al.[42]

If the published results by Jensens et al.[29] on prolongation of the rabbit papillary muscle can be translated to human heart muscle, (S)-1 is potentially a promising target in the fight against pathogens and cancer cells. However, more investigations on the enantiomers are needed to rule out side effects. Further biological tests will be carried out in the near future.

Acknowledgment

We would like to acknowledge Karoline Gangstad Primdahl for help with chiral HPLC analyses and Roland Kallenborn for lending us the chiral column.

• References and Notes

• 1 Fenton M, Rathbone J, Reilly J. Cochrane Database Syst. Rev. 2007; 3: CD001944
• 2 Judah L, Murphree O, Seager L. Am. J. Psychiatry 1959; 115: 1118
• 3 Kinross-Wright J. JAMA, J. Am. Med. Assoc. 1959; 170: 1283
• 4 Fletcher GF, Kazamias TM, Wenger NK. Am. Heart J. 1969; 78: 135
• 5 Harrigan EP, Miceli JJ, Anziano R, Watsky E, Reeves KR, Cutler NR, Sramek J, Shiovitz T, Middle M. J. Clin. Psychopharmacol. 2004; 24: 62
• 6 Hartigan-Go K, Bateman DN, Nyberg G, Martensson E, Thomas SH. L. Clin. Pharmacol. Ther. 1996; 60: 543
• 7 Salih IS. M, Thanacoody RH. K, McKay GA, Thomas SH. L. Clin. Pharmacol. Ther. 2007; 82: 548
• 8 Mehtonen OP, Aranko K, Malkonen L, Vapaatalo H. Acta Psychiatr. Scand. 1991; 84: 58
• 9 Ray WA, Meredith S, Thapa PB, Meador KG, Hall K, Murray KT. Arch. Gen. Psychiatry 2001; 58: 1161
• 10 Reilly JG, Ayis SA, Ferrier IN, Jones SJ, Thomas SH. L. Br. J. Psychiatry 2002; 180: 515
• 11 Hennessy S, Bilker WB, Knauss JS, Margolis DJ, Kimmel SE, Reynolds RF, Glasser DB, Morrison MF, Strom BL. Br. Med. J. 2002; 325: 1070
• 12 Ray WA, Chung CP, Murray KT, Hall K, Stein CM. N. Engl. J. Med. 2009; 360: 225
• 13 Reilly JG, Ayis SA, Ferrier IN, Jones SJ, Thomas SH. L. Lancet 2000; 355: 1048
• 14 Thanacoody RH. K. Recent Pat. Anti-Infect. Drug Discovery 2011; 6: 92
• 15 Abbate E, Vescovo M, Natiello M, Cufre M, Garcia A, Gonzalez Montaner P, Ambroggi M, Ritacco V, van Soolingen D. J. Antimicrob. Chemother. 2012; 67: 473
• 16 Dastidar SG, Kristiansen JE, Molnar J, Amaral L. Antibiotics 2013; 2: 58
• 17 Amaral L, Viveiros M. Int. J. Antimicrob. Agents 2012; 39: 376
• 18 Ordway D, Viveiros M, Leandro C, Bettencourt R, Almeida J, Martins M, Kristiansen JE, Molnar J, Amaral L. Antimicrob. Agents Chemother. 2003; 47: 917
• 19 Martins M, Bleiss W, Marko A, Ordway D, Viveiros M, Leandro C, Pacheco T, Molnar J, Kristiansen JE, Amaral L. In Vivo 2004; 18: 787
• 20 Stenger M, Behr-Rasmussen C, Klein K, Groennemose RB, Andersen TE, Klitgaard JK, Kolmos HJ, Lindholt JS. PLoS One 2017; e0173362
• 21 Daniel WA, Wojcikowski J. Toxicol. Appl. Pharmacol. 1999; 158: 115
• 22 Zhang C, Gong P, Liu P, Zhou N, Zhou Y, Wang Y. Oncol. Rep. 2017; 37: 1168
• 23 Sachlos E, Risueno RM, Laronde S, Shapovalova Z, Lee J.-H, Russell J, Malig M, McNicol JD, Fiebig-Comyn A, Graham M, Levadoux-Martin M, Lee JB, Giacomelli AO, Hassell JA, Fischer-Russell D, Trus MR, Foley R, Leber B, Xenocostas A, Brown ED, Collins TJ, Bhatia M. Cell 2012; 149: 1284
• 24 Cheng HW, Liang YH, Kuo YL, Chuu CP, Lin CY, Lee MH, Wu AT. H, Yeh CT, Chen EI. T, Whang-Peng J, Su CL, Huang CY. F. Cell Death Dis. 2015; 6: e1753
• 25 Yue H, Huang D, Qin L, Zheng Z, Hua L, Wang G, Huang J, Huang H. BioMed Res. Int. 2016; 6709828
• 26 Spengler G, Csonka A, Molnar J, Amaral L. Anticancer Res. 2016; 36: 5701
• 27 Svendsen CN, Froimowitz M, Hrbek C, Campbell A, Kula N, Baldessarini RJ, Cohen BM, Babb S, Teicher MH, Bird ED. Neuropharmacology 1988; 27: 1117
• 28 Jortani SA, Poklis A. J. Anal. Toxicol. 1993; 17: 374
• 29 Jensen AS, Pennisi CP, Sevcencu C, Christensen JB, Kristiansen JE, Struijk JJ. Eur. J. Pharmacol. 2015; 747: 7
• 30 Bourquin JP, Schwarb G, Gamboni G, Fischer R, Ruesch L, Guldimann S, Theus V, Schenker E, Renz J. Helv. Chim. Acta 1958; 41: 1072
• 31 Choi S, Haggart D, Toll L, Cuny GD. Bioorg. Med. Chem. Lett. 2004; 14: 4379
• 32 Bosque I, González-Gómez JC, Foubelo F, Yus M. J. Org. Chem. 2012; 77: 780
• 33 Patrick KS, Singletary JL. Chirality 1991; 3: 208
• 34 Mohammad T, Hawes EM, McKay G, Midha KK. J. Labelled Compd. Radiopharm. 1990; 28: 1087
• 35 Mohammad T, Midha KK, Hawes EM. J. Labelled Compd. Radiopharm. 1988; 25: 415
• 36 Flock S, Antonsen S, Gallantree-Smith H, Langseter AM, Skatteboel L, Stenstroem Y. Tetrahedron Lett. 2016; 72: 4518
• 37 Sun X.-W, Liu M, Xu M.-H, Lin G.-Q. Org. Lett. 2008; 10: 1259
• 38 Aggarwal VK, Barbero N, McGarrigle EM, Mickle G, Navas R, Suárez JR, Unthank MG, Yar M. Tetrahedron Lett. 2009; 50: 3482
• 39 Senter TJ, Schulte ML, Konkol LC, Wadzinski TE, Lindsley CW. Tetrahedron Lett. 2013; 54: 1645
• 40 Schlauderer F, Lammens K, Nagel D, Vincendeau M, Eitelhuber AC, Verhelst SH. L, Kling D, Chrusciel A, Ruland J, Krappmann D, Hopfner K.-P. Angew. Chem. Int. Ed. 2013; 52: 10384
• 41 Poulsen M. Ø, Klitgaard JK, Christensen JB, Kallipolitis BH, Kaatz GW, Plenge P, Fey SJ, Kristiansen JE. Am. J. Bioavailab. Bioequiv. 2018; 1: 1
• 42 N-Boc (S)-2-(1-methylpiperidin-2-yl)ethanal [(S)-13]: 2-Allyl piperidine (37 mg, 0.16 mmol) was dissolved in dioxane (9.5 mL) and the mixture stirred vigorously for 10 min. 2,6-Lutidine (0.038 mL, 2 equiv), OsO₄ in tert-butanol (2.5 wt%, 0.047 mL, 0.020 mol%), water (3.2 mL), and NaIO₄ (0.14 g, 0.65 mmol, 4 equiv) were added and the mixture was stirred for a further 5 h. The reaction was quenched by adding water (8 mL), CH₂Cl₂ (16 mL) was added and the phases were separated. The aqueous fraction was further extracted with CH₂Cl₂ (3 × 16 mL), and the combined organic phases were washed with brine (16 mL), dried over Na₂SO₄, filtered, concentrated under reduced pressure and purified by flash chromatography (SiO₂) to yield a colorless oil (36.3 mg, quantitative). [α] _d ²⁰ –51.7 (c = 1, CH₃Cl) {lit.⁴³ [α] _d ²⁰ –50.6 (c = 0.9, CH₃Cl)}; R_f = 0.31 (7:3 hex/EtOAc). ¹H NMR (400 MHz, CDCl₃): δ = 9.71 (dd, J = 3.3, 2.2 Hz, 1 H), 4.81 (d, J = 8.9 Hz, 1 H), 3.97 (d, J = 13.8 Hz, 1 H), 2.81–2.66 (m, 2 H), 2.51 (m, 1 H), 1.58 (m, 2 H), 1.42 (s, 10 H). ¹³C NMR (100 MHz, CDCl₃): δ = 200.80, 154.67, 79.90, 45.86, 44.64, 39.24, 28.88, 28.36, 25.21, 18.91. (S)-2-(1-Methylpiperidin-2-yl)ethan-1-ol [(S)-5]: To a solution of (S)-13 (0.95 g, 4.2 mmol) in tetrahydrofuran (10 mL) at 0 °C was added 2.0 M lithium aluminum hydride in tetrahydrofuran (0.28 mL, 0.56 mmol). The reaction was heated at 50 °C for 18 hours then cooled to 0 °C, when water (2.0 mL), 1 M aq. NaOH (2.0 mL) and water (2.0 mL) were added successively. The phases were separated, and the organic phase was filtered through a pad of Celite^®. The filtrate was concentrated under reduced pressure and distilled in vacuo to yield the product as a colorless oil of sufficient purity (0.53 g, 3.7 mmol, 88%). The hydrochloride salt was prepared for NMR comparison with the literature.³³ [α] _d ²⁰ –43.9 (c = 2.5, EtOH) {lit.³³ [α] _d ²⁰ –44.2 (c = 2.5, EtOH)} ¹H NMR (400 MHz, CDCl₃): δ = 4.81 (br s, 1 H), 3.78 (m, 1 H), 3.59 (m, 1 H), 2.80 (m, 1 H), 2.26 (s, 3 H), 2.10 (m, 1 H), 2.00 (dd, J = 11.8, 3.5 Hz, 1 H), 1.81 (m, 1 H), 1.71–1.36 (m, 7 H), 1.28–1.15 (m, 1 H); ¹³C NMR (100 MHz, CDCl₃): δ = 61.99, 59.95, 56.61, 42.99, 33.43, 29.47, 25.08, 24.01.2-(2-Chloroethyl)-l-methylpiperidine [(S)-6]: Thionyl chloride (0.49 g, 0.30 mL, 4.1 mmol) was added dropwise to a stirred solution of the alcohol (0.25 g, 1.7 mmol) in anhydrous chloroform (15 mL) cooled in an ice bath. After addition was complete, the solution was heated to reflux on a steam bath for 3 hours. Volatiles were removed under reduced pressure and the residual solid was dissolved in 25% aq. HC1 (13 mL), treated with activated charcoal and filtered. The solution was evaporated to dryness on a rotary evaporator and the solid obtained was recrystallized from acetone, giving colorless crystals of the hydrochloride salt of (S)-6 (0.26 g, quantitative yield). [α] _d ²⁰ –44 (c = 1, H₂O) {lit.³³ [α] _d ²⁰ –44.9 (c = 2, H₂O)}; mp 132–133 °C [lit.³³ 131–132 °C]. ¹H NMR (400 MHz, CDCl₃): δ = 3.67–3.49 (m, 2 H), 2.81 (m, J = 11.7, 3.7, 1.4 Hz, 1 H), 2.25 (s, 3 H), 2.15–2.07 (m, 3 H), 1.86 (m, 1 H), 1.74–1.62 (m, 2 H), 1.61–1.46 (m, 2 H), 1.35–1.19 (m, 2 H); ¹³C NMR (100 MHz, CDCl₃): δ = 61.44, 56.90, 42.94, 41.71, 35.98, 30.47, 25.61, 24.07.Thioridazine [(S)-1]: A stirred mixture of phenothiazine 7 (0.25 g, 1 mmol), finely powdered NaOH (0.16 g, 4 mmol) and toluene (25 mL, dried over activated 4Å molecular sieves) was heated to reflux for 5 h under N₂ atmosphere. The hydrochloride of 6 (0.125 g, 0.51 mmol) was added portionwise over a period of 1 h. The mixture was then heated to reflux for 3 h, cooled to 0 °C and the phases were separated. The organic phase was extracted with 25% aq. HC1 (2 × 50 mL). The aqueous phase was then basified with NaOH and extracted with CH₂Cl₂ (3 × 50 mL). The combined organic extracts were washed with water (25 mL), dried over Na₂SO₄, filtered and evaporated on a rotary evaporator. The resulting residue was purified on silica to give (S)-thioridazine (0.15 g, 80% yield). All spectroscopic data correspond to the data of the racemate. The specific rotation corresponds to that previously published.^{9 1}H NMR (400 MHz, CDCl₃): δ = 7.24–6.75 (m, 7 H), 4.05–3.75 (m, 2 H), 2.95–2.70 (m, 1 H), 2.57–2.37 (m, 3 H), 2.23–2.18 (m, 3 H), 2.15–1.99 (m, 3 H), 1.93–1.79 (m, 1 H), 1.78–1.66 (m, 2 H), 1.58 (qd, J = 9.2, 7.4, 4.3 Hz, 2 H), 1.50–1.36 (m, 1 H), 1.36–1.24 (m, 1 H). ¹³C NMR (100 MHz, CDCl₃): δ = 145.75, 145.00, 137.54, 127.56, 127.47, 127.22, 125.32, 122.57, 122.26, 120.80, 115.71, 114.61, 62.15, 56.94, 43.97, 43.17, 30.92, 30.05, 25.74, 24.21, 16.50. [α] _d ²⁰ –21 (c = 1, EtOH) {lit.³⁰ [α] _d ²⁰ –21±2 (c = 1, EtOH)]; The racemic reference was purchased from Sigma–Aldrich chemicals.
• 43 Shaikh TM, Sudalai A. Eur. J. Org. Chem. 2010; 3437