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Exp Clin Endocrinol Diabetes 2019; 127(06): 377-384
DOI: 10.1055/s-0042-101164
Article
© Georg Thieme Verlag KG Stuttgart · New York

Effect of Lipoic Acid on Serum Paraoxonase-1 and Paraoxonase-3 Protein Levels and Activities in Diabetic Rats

E. Ozgun
1   Medical Biochemistry, Trakya University, Edirne, Turkey
,
G. S. Ozgun
1   Medical Biochemistry, Trakya University, Edirne, Turkey
,
S. S. Gokmen
1   Medical Biochemistry, Trakya University, Edirne, Turkey
,
S. Eskıocak
1   Medical Biochemistry, Trakya University, Edirne, Turkey
,
N. Sut
2   Biostatistics and Medical Informatics, Trakya University, Edirne, Turkey
,
M. Akıncı
1   Medical Biochemistry, Trakya University, Edirne, Turkey
,
E. Goncu
3   Medical Genetics, Trakya University, Edirne, Turkey
,
E. Cakır
1   Medical Biochemistry, Trakya University, Edirne, Turkey
› Author Affiliations
Further Information

Publication History

received 29 September 2015
revised 09 December 2015

accepted 18 January 2016

Publication Date:
05 February 2016 (online)

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Abstract

The aim of the present study was to investigate the effect of streptozotocin-induced diabetes mellitus and lipoic acid treatment on serum paraoxonase-1 and paraoxonase-3 protein levels and paraoxonase, arylesterase and lactonase activities.

36 rats were equally and randomly divided into 4 groups as control, lipoic acid, diabetes and diabetes+lipoic acid. To induce diabetes, a single dose of streptozotocin (40 mg/kg) was injected intraperitoneally to diabetes and diabetes+lipoic acid groups. Lipoic acid (10 mg/kg/day) was injected intraperitoneally for 14 days to lipoic acid and diabetes+lipoic acid groups. Serum PON1 and PON3 protein levels were measured by western blotting. Serum paraoxonase, arylesterase and lactonase activities were determined by the measuring initial rate of substrate (paraoxon, phenylacetate and dihydrocoumarin) hydrolysis.

Streptozotocin-induced diabetes mellitus caused a significant decrease whereas lipoic acid treatment caused a significant increase in serum PON1 and PON3 protein levels and paraoxonase, arylesterase and lactonase activities. The increase percent of serum PON3 protein was higher than that of serum PON1 protein and the increase percent of serum lactonase activity was higher than that of serum paraoxonase and arylesterase activities in diabetes+lipoic acid group.

We can report that, like PON1 protein, PON3 protein and actually its lactonase activity may also have a role as an antioxidant in diabetes mellitus and lipoic acid treatment may be useful for the prevention of the atherosclerotic complications of diabetes by increasing serum PON1 and PON3 protein levels and serum enzyme activities.

Key words

streptozotocin-induced diabetes mellitus - lipoic acid - paraoxonase 1 - paraoxonase 3 - arylesterase - lactonase
 

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