Immunomodulatory Study of Different Solvent Extract of Elephantopus scaber Linn

Abstract

The aim and objective of present study is that to provide a scientific evidence for the immunomodulatory potential of Elephantopus scaber in vivo. The immunomodulatory activity was performed in petroleum ether, solvent ether, methanol and aqueous extract of aerial part and root of E. scaber by using models such as delayed type hypersensitivity, humoral antibody response to SRBC and carbon clearance test for phagocytic activity in Wistar rat. The observation and results of present study suggested that among the total 8 extract, only the petroleum ether and solvent ether aerial part extract of E. scaber significantly decreased in delayed type hypersensitivity (DTH), humoral antibody (HA) titre and phagocytic index thereby indicating significant immunosuppressant activity in experimental animals.

Key words

Elephantopus scaber - Immunomodulators - DTH - phagocytic index

• References

• 1 Patwardhan BK, Kalbag D, Patki PS. et al. Search of immunomodulatory agents: a review. Indian Drugs 1990; 28: 56-63
• 2 Kiritikar KD, Basu BD. Indian medicinal plants. 2nd ed. Deharadun: International Book Distributors; 1991: 1328-1329
• 3 Kurokawa T, Nakanishi K. Deoxyelephantopin and its interrelation with elephantopin. Tetrahedron Lett 1970; 33: 2863-2866
• 4 Taylor RS, Manandhar NP. Towers GHN. Screening of selected medicinal plants of Nepal for antimicrobial activities. J Ethnopharmacol 1995; 46: 153-159
• 5 Hui C, But PPH. Current advance in ethnopharmacology of ‘‘Kudidan’’ (Herba Elephantopi). Chin J Integr Med 1998; 4: 229-234
• 6 Singh SDJ, Krishna V, Mankani KL. et al. Wound healing activity of the leaf extracts and deoxyelephantopin isolated from Elephantopus scaber Linn. Ind J Pharmacol 2005; 37: 238-242
• 7 Than NN, Fotso S, Sevvana M. et al. Sesquiterpene lactones from Elephantopus scaber. Z Naturforsch 2005; 60: 200-204
• 8 Wright CI, Buren LV, Kroner CI. et al Herbal medicines as diuretics: a review of the scientific evidence. J Ethnopharmacol 2007; 114: 1-31
• 9 Hiradeve SM, Rangari VD. Antibacterial and antifungal activities of Elephantopus scaber Linn. Internat J. Biomed Res 2015; 6: 338-345
• 10 Kulkarni SK. Hand book of experimental pharmacology. 1^st Ed. Delhi: Vallabh Prakashan; 1987: 88-90
• 11 Ghosh MN. Fundamental of experimental pharmacology. 3rd Ed Kolkatta: Hilton & Co.; 2005: 196
• 12 Lagrange PH, Mackaness GB, Miller TE. Potential of T-cell-mediated immunity by selective suppression of antibody formation with cyclophosphamide. J Exp Med 1974; 139: 1529-1539
• 13 Doherty NS. Selective effects of immunosuppressive agents against the delayed hypersensitivity response and humoral response to sheep red blood cells in mice. Agents Act 1981; 11: 237-242
• 14 Bin-Hafeez BB, Haque R, Parvez S. et al. Immunomodulatory effects of fenugreek (Trigonella foenum graecum L.) extract in mice. Internat Immunopharmacol 2003; 3: 257-265
• 15 Mediratta PK, Sharma KK, Singh S. Evaluation of immunomodulatory potential of Ocimum sanctum seed oil and its possible mechanism of action. J Ethnopharmacol 2002; 80: 15-20
• 16 Ghule B, Murugananthan G, Nakhate P. et al. Immunositmulant effect of Capparis zeylanica Linn. Leaves. J Ethnopharmacol 2006; 108: 311-315
• 17 Hudson L, Hay FC. Practical immunology. London: Blackwell; 1980: 73-92
• 18 Dunnet CW. New table for multiple comparisons with a control biometrics. 1964; 20: 482-491
• 19 Askenase PW, Van Loveren H, Kraeuter-Kops S. et al. Defective elicitation of delayed-type hypersensitivity in W/Wv and SI/SId mast cell-deficient mice. J Immunol 1983; 131: 2687-2694
• 20 Abbas AK, Lichtman AH. Basic Immunology: functions and disorders of the immune system. Philadelphia: WB Saunders Company; 2001
• 21 Gupta A, Khajuria A, Singh J. et al. Immunomodulatory activity of biopolymeric fraction RLJ-NE-205 from Picrorhiza kurroa. Internat Immunopharmacol 2006; 6: 1543-1549
• 22 Fulzele SV, Satturwar PM, Joshi SB. et al. Study of the immunomodulatory activity of Haridradi ghrita in rats. Indian J Pharmacol 2003; 35: 51-54
• 23 Ghaisas MM, Sheikh SA, Despande AD. Evaluation of the immunomodulatory activity of ethanolic extract of the stem bark of Bauhinia variegate L. Int J Green pharmacy 2009; 3: 70-74