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Drug Res (Stuttg) 2017; 67(10): 576-582
DOI: 10.1055/s-0043-110484
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Cytoprotective Effects of Pumpkin (Cucurbita Moschata) Fruit Extract against Oxidative Stress and Carbonyl Stress

Reyhaneh Shayesteh
1   Zanjan Metabolic Diseases Research Center, Zajan University of Medical Sciences, Zanjan, Iran
2   Department of Pharmacology and Toxicology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
,
Mohammad Kamalinejad
3   Department of Pharmacognosy, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
,
Hasan Adiban
2   Department of Pharmacology and Toxicology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
,
Azin Kardan
2   Department of Pharmacology and Toxicology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
,
Fariborz Keyhanfar
4   Department of Pharmacology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
,
Mohammad Reza Eskandari
1   Zanjan Metabolic Diseases Research Center, Zajan University of Medical Sciences, Zanjan, Iran
2   Department of Pharmacology and Toxicology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
› Author Affiliations
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Publication History

Publication Date:
06 June 2017 (online)

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Abstract

Background Diabetes mellitus is a chronic endocrine disorder that is associated with significant mortality and morbidity due to microvascular and macrovascular complications. Diabetes complications accompanied with oxidative stress and carbonyl stress in different organs of human body because of the increased generation of free radicals and impaired antioxidant defense systems. In the meantime, reactive oxygen species (ROS) and reactive carbonyl species (RCS) have key mediatory roles in the development and progression of diabetes complications. Therapeutic strategies have recently focused on preventing such diabetes-related abnormalities using different natural and chemical compounds. Pumpkin (Cucurbita moschata) is one of the most important vegetables in the world with a broad-range of pharmacological activities such as antihyperglycemic effect.

Methods In the present study, the cytoprotective effects of aqueous extract of C. moschata fruit on hepatocyte cytotoxicity induced by cumene hydroperoxide (oxidative stress model) or glyoxal (carbonylation model) were investigated using freshly isolated rat hepatocytes.

Results The extract of C. moschata (50 μg/ml) excellently prevented oxidative and carbonyl stress markers, including hepatocyte lysis, ROS production, lipid peroxidation, glutathione depletion, mitochondrial membrane potential collapse, lysosomal damage, and cellular proteolysis. In addition, protein carbonylation was prevented by C. moschata in glyoxal-induced carbonyl stress.

Conclusion It can be concluded that C. moschata has cytoprotective effects in oxidative stress and carbonyl stress models and this valuable vegetable can be considered as a suitable herbal product for the prevention of toxic subsequent of oxidative stress and carbonyl stress seen in chronic hyperglycemia.

Key words

antioxidant - C. moschata - carbonylation - cytoprotection - diabetes - hepatoprotection
 

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