Download PDF
Plant Biol (Stuttg) 2000; 2(2): 176-181
DOI: 10.1055/s-2000-9154
Original Paper
Georg Thieme Verlag Stuttgart ·New York

Cytoskeletal Drugs and Gravity-Induced Lateral Auxin Transport in Rice Coleoptiles

R. Godbolé, W. Michalke, P. Nick 1 , and R. Hertel
  • Institut für Biologie III and 1II, Albert-Ludwigs-Universität Freiburg, Freiburg, Germany
Further Information

Publication History

November 10, 1999

November 23, 1999

Publication Date:
31 December 2000 (online)

   Permissions and Reprints

Abstract:

Gravity-induced events such as amyloplast sedimentation and lateral auxin transport were probed with cytoskeletal drugs in coleoptiles of rice (Oryza sativa L.). Amyloplast sedimentation was retarded by taxol. Lateral transport of auxin (3H-indoleacetic acid) was strongly inhibited by EPC (ethyl N-phenylcarbamate), but only partially inhibited by taxol. 1 mM EPC reduced gravitropism while phototropism was not affected. The findings suggest that microtubules may transduce pressure or proximity of amyloplasts to the auxin exporter in the plasmalemma.

Key words:

Amyloplasts - cytoskeletal drugs - gravitropism - lateral auxin transport - microtubules - rice (Oryza sativa L.)

References

  • 01 Abe,  K.,, Takahashi,  H.,, and Suge,  H.. (1994);  Graviresponding sites in shoots of normal and “lazy” rice seedlings.  Physiol. Plantarum. 92 371-374
    Search in Google Scholar
  • 02 Björkman,  T.. (1988);  Perception of gravity by plants.  Adv. Bot. Res.. 15 1-41
    Search in Google Scholar
  • 03 Blancaflor,  E. B., and Hasenstein,  K. H.. (1996);  Organization of cortical microtubules in graviresponding maize roots.  Planta. 191 230-237
    Search in Google Scholar
  • 04 Cande,  W. Z.,, Goldsmith,  M. H. M.,, and Ray,  P. M.. (1973);  Polar auxin transport and auxin-induced elongation in the absence of cytoplasmic streaming.  Planta. 111 279-296
    Search in Google Scholar
  • 05 Ding,  J. P., and Pickard,  B. G.. (1993);  Mechanosensory calcium-selective cation channels in epidermal cells.  Plant J.. 3 83-110
    Search in Google Scholar
  • 06 Dolk,  H. E.. (1936);  Geotropism and the growth substance.  Rec. Trav. bot. néerl.. 33 509-585
    Search in Google Scholar
  • 07 Godbolé,  R.,, Takahashi,  H.,, and Hertel,  R.. (1999);  The lazy mutation in rice affects a step between statoliths and gravity-induced lateral auxin transport.  Plant Biology. 1 379-381
    Search in Google Scholar
  • 08 Hertel,  R.,, de la Fuente,  R.,, and Leopold,  A. C.. (1969);  Geotropism and the lateral transport of auxin in the corn mutant amylomaize. .  Planta. 88 204-214
    Search in Google Scholar
  • 09 Iino,  M.,, Tarui,  Y.,, and Uematsu,  C.. (1996);  Gravitropism of maize and rice coleoptiles: dependence on the stimulation angle.  Plant Cell Environ.. 19 1160-1168
    Search in Google Scholar
  • 10 Kiss,  J. Z.,, Hertel,  R.,, and Sack,  F. D.. (1989);  Amyloplasts are necessary for full gravitropic sensitivity in roots of Arabidopsis thaliana. .  Planta. 177 198-206
    CrossrefPubMedSearch in Google Scholar
  • 11 Kuznetsov,  O. A., and Hasenstein,  K. H.. (1996);  Intracellular magnetophoresis of amyloplasts and induction of root curvature.  Planta. 198 87-94
    PubMedSearch in Google Scholar
  • 12 Masson,  P. H.. (1995);  Root gravitropism.  BioEssays. 17 119-127
    CrossrefPubMedSearch in Google Scholar
  • 13 Mizuno,  K., and Suzaki,  T.. (1990);  Effects of anti-microtubule drugs on in vitro polymerization of tubulin from mung bean.  Bot. Mag. Tokyo. 103 435-448
    PubMedSearch in Google Scholar
  • 14 Morejohn,  L. C.. (1991) The molecular pharmacology of plant tubulin and microtubules.  The Cytoskeletal Basis of Plant Growth and Form. Lloyd C. W., ed. London; Academic Press 43
    Search in Google Scholar
  • 15 Morgan,  D. G.. (1964);  Influence of NPA on the movement of IAA in plants.  Nature. 201 476-477
    PubMedSearch in Google Scholar
  • 16 Neumann,  R., and Iino,  M.. (1997);  Phototropism of rice (Oryza sativa L.) coleoptiles: fluence-response relationships, kinetics and photogravitropic equilibrium.  Planta. 201 288-292
    CrossrefPubMedSearch in Google Scholar
  • 17 Nick,  P.,, Schäfer,  E.,, Hertel,  R.,, and Furuya,  M.. (1991);  Putative role of microtubules in gravitropism of maize coleoptiles.  Plant Cell Physiol.. 32 873-880
    PubMedSearch in Google Scholar
  • 18 Nick,  P., and Furuya,  M.. (1993);  Phytochrome-dependent decrease of gibberellin sensitivity.  Plant Growth Regul.. 12 195-206
    CrossrefPubMedSearch in Google Scholar
  • 19 Nick,  P.,, Yatou,  O.,, Furuya,  M.,, and Lambert,  A. M.. (1994);  Auxin-dependent microtubule responses and seedling development are affected in a rice mutant resistant to EPC.  Plant J.. 6 651-663
    CrossrefPubMedSearch in Google Scholar
  • 20 Nick,  P.,, Lambert,  A. M.,, and Vantard,  M.. (1995);  A microtubule-associated protein in maize is expressed during phytochrome-induced cell elongation.  Plant J.. 8 835-844
    PubMedSearch in Google Scholar
  • 21 Parker,  K. E., and Briggs,  W. R.. (1990);  Transport of indole-3-acetic acid during gravitropism in intact maize coleoptiles.  Plant Physiol.. 94 1763-1768
    PubMedSearch in Google Scholar
  • 22 Parness,  J., and Horwitz,  S. B.. (1981);  Taxol binds to polymerized tubulin in vitro. .  J. Cell Biol.. 91 479-487
    CrossrefPubMedSearch in Google Scholar
  • 23 Rowinsky,  E. K.,, Cazenave,  L. A.,, and Donehower,  F. C.. (1991);  Taxol: a novel investigational antimicrotubule agent.  J. Natl. Cancer Inst.. 82 1247-1259
    PubMedSearch in Google Scholar
  • 24 Sack,  F. D.. (1991);  Plant gravity sensing.  Int. Rev. Cytol.. 127 193-252
    PubMedSearch in Google Scholar
  • 25 Sack,  F. D.. (1997);  Plastids and gravitropic sensing.  Planta. 203 68
    PubMedSearch in Google Scholar
  • 26 Schwuchow,  J.,, Sack,  F. D.,, and Hartmann,  E.. (1990);  Microtubule distribution in gravitropic protonemata of the moss Ceratodon. .  Protoplasma. 159 60-69
    CrossrefPubMedSearch in Google Scholar
  • 27 Sedbrook,  J.,, Chen,  R.,, and Masson,  P. H.. (1999);  ARG1 (altered response to gravity) encodes a DnaJ-like protein that potentially interacts with the cytoskeleton.  Proc. Natl. Acad. Sci. USA. 96 1140-1145
    CrossrefPubMedSearch in Google Scholar
  • 28 Sievers,  A., and Hejnowicz,  Z.. (1994);  Graviperzeption und das Cytoskelett.  Acta Leopold.. 40 257-266
    PubMedSearch in Google Scholar
  • 29 Staves,  M. P.,, Wayne,  R.,, and Leopold,  A. C.. (1997);  Cytochalasin D does not inhibit gravitropism in roots.  Am. J. Bot.. 84 1530-1535
    PubMedSearch in Google Scholar
  • 30 Thomson,  K. St.,, Hertel,  R.,, Müller,  S.,, and Tavares,  J. E.. (1973);  1-Naphthylphthalamic acid and 2,3,5-triiodobenzoic acid: In vitro binding to particulate cell fractions and action on auxin transport in corn coleoptiles.  Planta. 109 337-352
    CrossrefPubMedSearch in Google Scholar
  • 31 Trewavas,  A. J.. (1992);  What remains of the Cholodny-Went theory¿- A summing up.  Plant Cell Environ.. 15 793-794
    PubMedSearch in Google Scholar
  • 32 Wacker,  I.,, Quader,  H.,, and Schnepf,  H.. (1998);  Influence of the herbicide oryzalin on cytoskeleton and growth of Funaria hygrometrica protonemata.  Protoplasma. 142 55-67
    PubMedSearch in Google Scholar
  • 33 Walker,  L. M., and Sack,  F. D.. (1990);  Amyloplasts as possible statoliths in gravitropic protonemata of the moss Ceratodon purpureus. .  Planta. 181 71-77
    PubMedSearch in Google Scholar
  • 34 Wang,  Q. Y., and Nick,  P.. (1998);  The auxin response of actin is altered in the rice mutant Yin-Yang. .  Protoplasma. 204 22-33
    CrossrefPubMedSearch in Google Scholar
  • 35 Yamamoto,  E.,, Zeng,  L.,, and Baird,  V.. (1998);  Tubulin missense mutations correlate with antimicrotubule drug resistance in Eleusine indica. .  Plant Cell. 10 297-308
    CrossrefPubMedSearch in Google Scholar

R. Hertel

Institut für Biologie III and II Albert-Ludwigs-Universität Freiburg

Schänzlestr. 1

79104 Freiburg

Germany

Section Editor: M. Koornneef

Email: hertel@uni-freiburg.de