Abstract
High frequency of plant regeneration from Paulownia elongata was obtained on Murashige and Skoog (MS) medium and Woody Plant Medium (WPM), with
appropriate supplements of growth regulators. Leaves, leaves with petioles, internodes
and nodes excised from 3-month-old non-aseptically grown P. elongata were used as explants. The highest shoot regeneration efficiency (93.7 %) was obtained
from the nodes of P. elongata on MS medium supplemented with 0.1 mg/ml naphthaleneacetic acid (NAA) and 1 mg/ml
6-benzylaminopurine (BAP). The highest root formation efficiency (100 %) from the
regenerated shoots was obtained on WPM supplemented with 1 mg/ml indolebutyric acid
(IBA). Rooted plantlets were transplanted to soil with a survival efficiency of almost
100 %. The regeneration system reported here could be useful for rapid multiplication
of elite genotypes of P. elongata in a short period of time.
Abbreviations
BAP: 6-benzylaminopurine
IAA: indoleacetic acid
IBA: indolebutyric acid
MS: Murashige Skoog
NAA: naphthaleneacetic acid
WPM: Woody Plant Medium
Key words
Paulownia elongata
- micropropagation - nodal explant - tissue culture
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29P
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N. Gozukirmizi
TUBITAK, Marmara Research Center
Research Institute for Genetic Engineering and Biotechnology
P.O. Box 21
41470 Gebze-Kocaeli
Turkey
Email: nermin@rigeb.gov.tr
Section Editor: A. M. C. Emons
