Effect of Hyperthermic Preconditioning on the Survival of Ischemia-Reperfused Skin Flaps: A New Skin-Flap Model in the Mouse

Trinh Cao Minh; Shigeru Ichioka; Takashi Nakatsuka; Junsuke Kawai; Masahiro Shibata; Joji Ando; Kiyonori Harii

doi:10.1055/s-2002-19892

Journal of Reconstructive Microsurgery, Table of Contents

J Reconstr Microsurg 2002; 18(2): 115-120
DOI: 10.1055/s-2002-19892

Effect of Hyperthermic Preconditioning on the Survival of Ischemia-Reperfused Skin Flaps: A New Skin-Flap Model in the Mouse

Trinh Cao Minh¹ , Shigeru Ichioka² , Takashi Nakatsuka² , Junsuke Kawai¹ , Masahiro Shibata³ , Joji Ando³ , Kiyonori Harii¹

¹Department of Plastic and Reconstructive Surgery, University of Tokyo, Tokyo, Japan
²Department of Plastic and Reconstructive Surgery, Saitama Medical School, Saitama, Japan
³Department of Biomedical Engineering, University of Tokyo, Japan

Abstract

ABSTRACT

To investigate whether hyperthermic preconditioning can actually protect skin flaps against ischemia/reperfusion injury, the authors first developed a new skin-flap model in 15 mice, a dorsal bipedicle island skin-flap model. Then, another 75 mice were separated into five groups. Mice in Groups 1 to 4 received the same hyperthermic preconditioning, but had different recovery times of 6 hr, 24 hr, 48 hr, and 72 hr, respectively. Mice in Group 5 served as control. Island skin flaps were elevated in all groups, and then were subjected to 8 hr of ischemia and subsequent reperfusion. Flap survival was statistically significantly higher than in controls in animals in Groups 1 and 3, with recovery times of 6 hr and 48 hr, respectively. Mice in Groups 2 and 4 had recovery times of 24 hr and 72 hr, respectively. Hyperthermic preconditioning could thus protect skin flaps against ischemia/reperfusion injury, and there were two optimal periods for such a protective effect.

KEYWORD

Mouse - skin flap - hyperthermic preconditioning - heat shock

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References

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