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Horm Metab Res 2002; 34(2): 62-66
DOI: 10.1055/s-2002-20527
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© Georg Thieme Verlag Stuttgart · New York

Formation of Compound 305 Requires the Simultaneous Generation of Both Alloxan and GSH Radicals

H.  J.  Brömme 1 , W.  Mörke 2 , R.  Weinandy 3 , D.  Peschke 4 , E.  Peschke 4
  • 1 Institute of Pathophysiology, Martin Luther University, Halle-Wittenberg, Germany
  • 2 Institute of Analytic and Environmental Chemistry, Martin Luther University, Halle-Wittenberg, Germany
  • 3 Institute of Zoology, Martin Luther University, Halle-Wittenberg, Germany
  • 4 Institute of Anatomy and Cell Biology, Martin Luther University, Halle-Wittenberg, Germany
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Publication History

18 June 2001

11 October 2001

Publication Date:
04 March 2002 (online)

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Abstract

This in vitro study investigates the conditions under which “compound 305” is formed. Using HPLC, ESR as well as UV spectroscopy, “compound 305” was largely separated and characterized. It has an absorption peak at 314 nm, which changes after reoxygenation to shorter wavelengths within hours. The retention time of “compound 305” amounts to 10.93 ± 0.042 min. The formation of “compound 305” does not depend on alloxan (ALX) or reduced glutathione (GSH), but most likely on the steady-state concentration of the paramagnetic derivatives of both reactants (ALX· and GS·). The alloxan radical (ALX·) is formed by either a one-electron transfer from e. g. GSH to alloxan or oxidation of dialuric acid. The concentration of the ALX· was determined to be 12 ± 3.6 µmol/l using the stable ultramarine radical as an ESR standard. ALX· is stable only under anaerobic conditions. It disappears within 2 min in air. Since formation of “compound 305” needs both ALX· as well as GS·, which are also necessary for the generation of reactive oxygen species (ROS), it is assumed that formation of “compound 305” diminishes the toxicity of alloxan.

Key words

Alloxan - GSH - Radical - “Compound 305” - ESR - HPLC

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E. Peschke

Institute of Anatomy and Cell Biology · Martin Luther University Halle-Wittenberg ·

Grosse Steinstrasse 52 · 06097 Halle/Saale · Germany

Phone: + 49 (345) 557 1709 ·

Fax: + 49 (345) 557 4053

Email: elmar.peschke@medizin.uni-halle.de

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