Effects of Estradiol and Progesterone on Vertebral Collagen, Glycosaminoglycans and Phosphatases in Ovariectomized Adult Rats

Abstract

Vertebral collagen, glycosaminoglycans (GAGs), tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) were measured in ovariectomized (ovx) adult Wistar rats treated with estradiol (E₂) (10 µg/kg BW for 35 days on alternate days, and progesterone (P₄) (140 µg/kg BW for 35 days on alternate days) in E₂ + P₄ treated rats. P₄ given alone or in combination with E₂ significantly increased the levels of collagen in the vertebral bone. Neither ovx nor E₂ treatment altered the concentration of collagen in these rats. Administration of E₂ or P₄ significantly decreased the concentration of hyaluronic acid (HA), but remaining unaffected when a combination of these steroids was given. In contrast to their effect on HA, E₂ and P₄ each significantly increased the levels of chondroitin sulfate (CS) in the vertebral bone. The specific activity of ALP was decreased after ovx. E₂ and P₄ alone or in combination also registered a significant decrease in the activities of ALP and TRAP. The results suggest that E₂ and P₄ each exert definite effects on vertebral bone turnover in ovariectomized rats.

Key words

Bone - Collagen - Estradiol - Glycosaminoglycans - Ovariectomy - Phosphatases - Progesterone

References

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