The Genus Artemisia and its Allies: Phylogeny of the Subtribe Artemisiinae (Asteraceae, Anthemideae) Based on Nucleotide Sequences of Nuclear Ribosomal DNA Internal Transcribed Spacers (ITS)

J. Vallès; M. Torrell; T. Garnatje; N. Garcia-Jacas; R. Vilatersana; A. Susanna

doi:10.1055/s-2003-40790

Plant Biology, Table of Contents

Plant Biol (Stuttg) 2003; 5(3): 274-284
DOI: 10.1055/s-2003-40790

Original Paper

Georg Thieme Verlag Stuttgart · New York

The Genus Artemisia and its Allies: Phylogeny of the Subtribe Artemisiinae (Asteraceae, Anthemideae) Based on Nucleotide Sequences of Nuclear Ribosomal DNA Internal Transcribed Spacers (ITS)

J. Vallès¹ , M. Torrell¹ , T. Garnatje² , N. Garcia-Jacas² , R. Vilatersana² , A. Susanna²

¹Laboratori de Botànica, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Catalonia, Spain
²Institut Botànic, Barcelona, Catalonia, Spain

Abstract

Sequences of the internal transcribed spacers (ITS1 and ITS2) of nuclear ribosomal DNA were analysed for 44 Artemisia species (46 populations) representing all the five classical subgenera and the geographical range of the genus, 11 species from 10 genera closely related to Artemisia, and six outgroup species from five other genera of the Anthemideae. The results definitely support the monophyly of the genus Artemisia in its broadest sense (including some taxa segregated as independent genera, like Oligosporus and Seriphidium). Eight main clades are established in this molecular phylogeny within Artemisia; they agree in part with the classical subdivision of the genus, but they also suggest that some infrageneric groups must be redefined, especially the subgenus Artemisia. The subgenera Tridentatae and Seriphidium are independent from each other. Some of the satellite genera are clearly placed within Artemisia (Artemisiastrum, Filifolium, Mausolea, Picrothamnus, Sphaeromeria, Turaniphytum), whereas some others fall outside the large clade formed by this genus (Brachanthemum, Elachanthemum, Hippolytia, Kaschgaria). Our results, correlated to other data such as pollen morphology, allow us to conclude that the subtribe Artemisiinae as currently defined is a very heterogeneous group. Affinities of the largest genus of the subtribe and tribe, Artemisia, and of other genera of the subtribe to some genera from other subtribes of the Anthemideae strongly suggest that subtribe Artemisiinae needs a deep revision and redefinition. Phylogenetic utility of region trnL-F of the plastid DNA in the genus Artemisia and allies was also evaluated: sequences of the trnL-F region in Artemisia do not provide phylogenetic information.

Key words

Artemisia - Artemisiinae - Anthemideae - Asteraceae - Compositae - nrDNA ITS - molecular phylogeny.

Full Text

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J. Vallès

Laboratori de Botànica
Facultat de Farmàcia
Universitat de Barcelona

Av. Joan XXIII, s/n

08028 Barcelona

Catalonia, Spain

Email: avalles@farmacia.far.ub.es

Section Editor: F. Salamini