Abstract
A new and efficient method for preparation of the diastereomers of 5′-O -DMT-nucleoside 3′-O -methanephosphonothioates has been elaborated based on an oxathiaphospholane approach, developed earlier in this laboratory. Appropriately protected 3′-OH nucleosides react in the presence of DBU with 2-methyl-2-thio-1,3,2-oxathiaphospholane providing an equimolar mixture of diastereomers of nucleoside 3′-O -methanephosphonothioates which were separated by silica gel column chromatography.
Key words
antisense agents - antiviral agents - nucleoside - oxathiaphospholane - methanephosphonothioate
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