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Plant Biol (Stuttg) 2006; 8(5): 715-722
DOI: 10.1055/s-2006-924174
Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Colleters in Bathysa nicholsonii K. Schum. (Rubiaceae): Ultrastructure, Secretion Protein Composition, and Antifungal Activity

E. de Castro Miguel1 , V. Moreira Gomes2 , M. A. de Oliveira3 , M. Da Cunha1
  • 1Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego, 2000, 28013-602 Campos dos Goytacazes, RJ, Brazil
  • 2Laboratório de Fisiologia e Bioquímica de Microrganismos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego, 2000, 28013-602 Campos dos Goytacazes, RJ, Brazil
  • 3Instituto de Pesquisa e Desenvolvimento, UNIVAP - Universidade do Vale do Paraíba, Av. Shishima Hifumi, 2911, Bairro Urbanova, 12244-000 São José dos Campos, SP, Brazil
Further Information

Publication History

Received: January 4, 2006

Accepted: March 29, 2006

Publication Date:
24 July 2006 (online)

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Abstract

Colleters are secretory structures well distributed in many organs of Angiosperms. Ultrastructurally, the colleters secretory cell presents an enhanced endoplasmic reticulum, Golgi apparatus, and mitochondria. Secretion synthesis, transportation, and passage through outer cell wall is poorly characterized. This study characterized the anatomy and ultrastructure of Bathysa nicholsonii (Rubiaceae) colleters and evaluated the presence of protein in the secretion and its antifungal property. Samples were collected and prepared according to usual techniques in light and electron microscopy, electrophoresis, and fungal growth inhibition assay. Colleters are of a standard type, cylindrical and elongated, formed by one secretory epidermal palisade layer, and a central axis formed by parenchymatic cells and a vascular trace. Epidermal cells have dense cytoplasm with abundant ribosome, a nucleus, enhanced endoplasmic reticulum and Golgi apparatus. The outer cell wall presented morphologically distinct layers. The presence of secretory cavities was noted in all outer cell wall extents. Secretion preparations analyzed by SDS-PAGE showed that B. nicholsonii secretion is a mixture of proteins with molecular masses covering a range of approximately 66 to 24 kDa. This preparation presented an inhibitory effect on the fungi spore growth.

Key words

Secretory structure - microscopy - plant anatomy - ultrastructure - development - Bathysa - Rubiaceae - colleter.

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M. Da Cunha

Laboratório de Biologia Celular e Tecidual
Centro de Biociências e Biotecnologia
Universidade Estadual do Norte Fluminense

Av. Alberto Lamego, 2000

28013-602 Campos dos Goytacazes, RJ

Brazil

Email: maura@uenf.br

Editor: N. M. van Dam