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Plant Biol (Stuttg) 2007; 9(1): 101-108
DOI: 10.1055/s-2006-924455
Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Genetic Diversity of Picea asperata Populations Based on RAPDs

X. Xue1 , 2 , Y. Wang1 , 2 , H. Korpelainen3 , C. Li1
  • 1Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, Chengdu 610041, China
  • 2Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
  • 3Department of Applied Biology, University of Helsinki, P.O. Box 27, 00014 Helsinki, Finland
Further Information

Publication History

Received: December 8, 2005

Accepted: June 14, 2006

Publication Date:
28 September 2006 (online)

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Abstract

The genetic diversity of ten natural populations of Picea asperata Mast. were studied using RAPD markers. A total of 160 reproducible fragments were produced from the ten primers used. The mean number of fragments detected per individual was 114.7. Altogether 120 fragments were polymorphic among the ten populations, none of them were found to be population-specific. Nei's expected heterozygosity (He) ranged from 0.233 to 0.269, and the average was 0.247. The analysis of molecular variance revealed that the coefficient of gene differentiation among populations, based on FST and the unbiased estimate Φst, equaled 0.224 and 0.290, respectively. Such high values indicate that there is significant differentiation among populations, which could result from several factors, including restricted gene flow between populations (Nm = 0.866). Founder events may be another factor attributing to the high level of genetic differentiation. In addition, it was discovered that the geographic distribution is not correlated with the genetic distances among the populations of P. asperata.

Key words

Genetic diversity - Picea asperata Mast. - RAPD.

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C. Li

Chengdu Institute of Biology
Chinese Academy of Sciences

P.O. Box 416

Chengdu 610041

China

Email: licy@cib.ac.cn

Editor: M. Koornneef

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