Int J Sports Med 2008; 29(1): 27-33
DOI: 10.1055/s-2007-965072
Physiology & Biochemistry

© Georg Thieme Verlag KG Stuttgart · New York

Changes of Urinary 8-Hydroxydeoxyguanosine Levels during a Two-Day Ultramarathon Race Period in Japanese Non-Professional Runners

M. Miyata1 , H. Kasai2 , K. Kawai2 , N. Yamada3 , M. Tokudome4 , H. Ichikawa1 , C. Goto5 , Y. Tokudome5 , K. Kuriki6 , H. Hoshino7 , K. Shibata8 , S. Suzuki1 , M. Kobayashi9 , H. Goto9 , M. Ikeda10 , T. Otsuka9 , S. Tokudome1
  • 1Department of Health, Promotion, and Preventive Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
  • 2Department of Environmental Oncology, University of Occupational and Environmental Health, Kitakyushu, Japan
  • 3Department of Applied Clinical Dietetics, Kitasato University of Medical Technology, Yamato-cho, Japan
  • 4Department of Rehabilitation, Yohohama City University Medical Center, Yokohama, Japan
  • 5Department of Health and Nutrition, Nagoya Bunri University, Inazawa, Japan
  • 6Department of Epidemiology and Prevention, Aichi Cancer Center Research Institute, Nagoya, Japan
  • 7Department of Early Childhood Studies, Aichi Bunkyo Women's College, Inazawa, Japan
  • 8Department of Health Maintenance, Kasugai Health Maintenance Center, Kasugai, Japan
  • 9Department of Musculoskeletal Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
  • 10Department of Occupational Health Economics, University of Occupational and Environmental Health, Kitakyushu, Japan
Further Information

Publication History

accepted after revision October 10, 2006

Publication Date:
05 July 2007 (online)

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Abstract

Using the urinary 8-hydroxydeoxyguanosine (8-OHdG) concentration, effects of participation in a two-day ultramarathon race period on oxidative DNA damage were investigated in Japanese nonprofessional runners. Before the first day (baseline), after the first day (mid-race) of 40-km running, and after the second day (post-race) of 90 km running, biomaterials were successfully sampled from 95 participants (males, 79; females, 16) who completed the full race. We analyzed urine for 8-OHdG and blood for aspartate aminotransferase (AST), creatine phosphokinase (CPK) and myoglobin, and evaluated fluctuation in the values at three sampling time points. Adjusted baseline urinary 8-OHdG levels (µg/g creatinine) (mean ± standard deviation) showed no significant differences between males and females, at 2.85 ± 1.17 and 3.04 ± 1.56, respectively. In males, mid-race urinary 8-OHdG levels rose to 3.29 ± 1.15 (p < 0.01), but then returned to 2.73 ± 1.16 at the post-race time point (p < 0.01). In females, a similar increase to 3.32 ± 1.47 and subsequent decline to 2.80 ± 1.47 were noted. In contrast, AST, CPK and myoglobin were increased at both mid- and post-time points and particularly the latter, independent of the sex. Extreme prolonged exercise in a two-day ultramarathon race period causes oxidative DNA damage but antioxidant repair systems are apparently induced to protect against oxidative DNA stress with physical exercise.

References

Prof. Dr. Shinkan Tokudome

Department of Health, Promotion, and Preventive Medicine
Nagoya City University
Graduate School of Medical Sciences

1 Kawasumi, Mizuho-cho

Mizuho-ku

467-8601 Nagoya

Japan

Phone: + 81 5 28 53 81 74

Fax: + 81 5 28 42 38 30

Email: tokudome@med.nagoya-cu.ac.jp