Exp Clin Endocrinol Diabetes 2008; 116(6): 309-314
DOI: 10.1055/s-2008-1042407
Article

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Decreased Thrombospondin- I (TSP-I) Expression in the Hippocampus of Streptozotocin-induced Diabetic Rats

X.-G. Zhang 1 , H. Yan 2 , Y.-L. Shen 3 , X.-M. Zhang 4
  • 1Department of Clinical Pharmacology, The First Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China
  • 2Department of Basic Medicine, Zhejiang University School of Medicine, Hangzhou, China
  • 3Department of Physiology, Zhejiang University School of Medicine, Hangzhou, China
  • 4Department of Anatomy and Cell Biology, Zhejiang University School of Medicine, Hangzhou, China
Further Information

Publication History

received 30.10.2007 first decision 03.12.2007

accepted 09.01.2008

Publication Date:
17 March 2008 (online)

Abstract

Diabetes mellitus (DM) may give rise to cognitive impairment, but the pathological mechanism involved was still unknown. We investigated the thrombospondin-I (TSP-I) expression level in hippocampus of streptozotocin-induced diabetic rats, which, as a matricellular, calcium-binding protein that participates in cellular responses to growth factors, cytokines and injury, has been indicated as important synaptogenic components recently. We employed 20 streptozotocin (STZ)-induced diabetic rats. The weight, blood sugar and urine sugar were measured before and after model induction in diabetes and normal groups. We did immunohistochemical localization of TSP-I and RT-PCR was applied to determine TSP-I mRNA level in the hippocampus of both groups. Moreover, transmission electron microscope (TEM) was used to study the ultrastuctural changes of the hippocampus. All data were analyzed by the independent samples t-test. We found that the expression of TSP-I markedly decreased in the hippocampal neuronal cells. Moreover, TEM results showed the ultrastructures of diabetic hippocampus, including area CA1 and DG, neurons were characterized by mitochondria swelling, increased heterochromatin accumulation and reduced synaptic contacts. The present study provides experimental evidences that decreased TSP-I expression may help to explain the reduced synaptogenesis and altered hippocampal ultrastucture, both of which may contribute to the pathogenesis of diabetic dementia.

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Grant support: Natural Science Foundation of Zhejiang Province (No. Y204036) Fund of Health bureau of Zhejiang Province (No. 2006A083).

Correspondence

X.-M. Zhang

Zhejiang University School of Medicine

310058 Hangzhou

China

Phone: +86/571/8820 80 62

Fax: +86/571/8820 80 62

Email: zxm@zju.edu.cn

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