Abstract
The molecular mechanism of insulin resistance induced by high-fructose feeding is
not fully understood. The present study investigated the role of downstream signaling
molecules of phosphatidylinositol 3-kinase (PI3K) in the insulin-stimulated skeletal
muscle of high-fructose-fed rats. Rats were divided into chow-fed and fructose-fed
groups. The results of the euglycemic clamp study (insulin infusion rates: 6 mU/kg
BW/min) showed a significant decrease in the glucose infusion rate (GIR) and the metabolic
clearance rate of glucose (MCR) in fructose-fed rats compared with chow-fed rats.
In skeletal muscle removed immediately after the clamp procedure, high-fructose feeding
did not alter protein levels of protein kinase B (PKB/Akt), protein kinase C ζ (PKCζ),
or glucose transporter 4 (GLUT4). However, insulin-stimulated phosphorylation of Akt
and PKCζ and GLUT4 translocation to the plasma membrane were reduced. Our findings
suggest that insulin resistance in fructose-fed rats is associated with impaired Akt
and PKCζ activation and GLUT4 translocation in skeletal muscle.
Key words
insulin resistance - euglycemic clamp - protein kinase - glucose transporter - insulin
signaling
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Correspondence
Y. OshidaMD, PhD
Department of Sports Medicine
Graduate School of Medicine
Nagoya University
464-8601 Nagoya
Japan
Phone: +81/52/789 39 61
Fax: +81/52/789 39 57
Email: oshida@htc.nagoya-u.ac.jp
