Effect of plasminogen activator inhibitor-1 deficiency on nutritionally-induced obesity in mice

Roger H. Lijnen

doi:10.1160/TH05-01-0043

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2005; 93(05): 816-819
DOI: 10.1160/TH05-01-0043

Rapid and Short Communication

Schattauer GmbH

Effect of plasminogen activator inhibitor-1 deficiency on nutritionally-induced obesity in mice

Roger H. Lijnen

¹Center for Molecular and Vascular Biology, KU Leuven, Belgium

› Author Affiliations

Abstract

Summary

Plasminogen activator inhibitor-1 (PAI-1) is the main physiological inhibitor of tissue-type (t-PA) and urokinase-type (u-PA) plasminogen activator. Recent studies in murine models have yielded apparently conflicting data on a potential role of PAI-1 in adipose tissue development and obesity. To reinvestigate this issue, we have rederived PAI-1 deficient (PAI-1^-/-) and wild-type (WT) mice and generated true littermates in a 81.25% C57Bl/6: 18.75% 129 SV genetic background. Male 5-week-old PAI-1^-/- and WT mice were kept on a high fat diet (20.1 kJ/g) for 15 weeks. Body weight gain was comparable for both genotypes, and at the time of sacrifice total body weights (39 ± 1.1 versus 41 ± 1.2 g) as well as the weights of subcutaneous (SC, 1,520 ± 110 versus 1,480 ± 110 mg) adipose tissue were not significantly different. In contrast, the gonadal (GON, 1,900 ± 43 versus 1,510 ± 86 mg, p < 0.005) tissue mass was larger in PAI-1^-/- mice. Plasma levels of insulin, leptin, glucose, triglycerides, total, HDL and LDL cholesterol were comparable for both genotypes. Immunohisto-chemical analysis of SC and GON adipose tissues did not reveal differences in adipocyte size or number between both genotypes, whereas blood vessel density was also comparable for GON fat but lower in SC fat of WT mice. Thus, this study in littermate mice on high fat diet did not reveal an effect of PAI-1 deficiency on body weight, and a differential effect on SC and GON adipose tissue.

Keywords

PAI-1 - obesity - adipose tissue - insulin

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References

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