The contribution of systems biology and reverse genetics to the understanding of Kaposi’s sarcoma-associated herpesvirus pathogenesis in endothelial cells

Michael Stürzl; Andreas Konrad; Khaled R. Alkharsah; Ramona Jochmann; Mathias Thurau; Gaby Marquardt; Thomas F. Schulz

doi:10.1160/TH09-07-0472

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2009; 102(06): 1117-1134
DOI: 10.1160/TH09-07-0472

Theme Issue Article

Schattauer GmbH

The contribution of systems biology and reverse genetics to the understanding of Kaposi’s sarcoma-associated herpesvirus pathogenesis in endothelial cells

Michael Stürzl

¹Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen-Nuremberg, Erlangen, Germany

,

Andreas Konrad

¹Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen-Nuremberg, Erlangen, Germany

,

Khaled R. Alkharsah

²Department of Virology, Medical School Hannover, Hannover, Germany

,

Ramona Jochmann

¹Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen-Nuremberg, Erlangen, Germany

,

Mathias Thurau

¹Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen-Nuremberg, Erlangen, Germany

,

Gaby Marquardt

¹Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen-Nuremberg, Erlangen, Germany

,

Thomas F. Schulz

²Department of Virology, Medical School Hannover, Hannover, Germany

› Author Affiliations

Abstract

Summary

Kaposi’s sarcoma-associated herpesvirus (KSHV) / human herpesvirus-8 is the causative agent of the endothelial cell-derived tumour Kaposi’s sarcoma. Herpesviruses possess large complex genomes which provide many options to regulate cellular physiology during the viral life cycle and in the course of tumourigenicity. Novel techniques of systems biology and reverse genetics are increasingly applied to dissect the complex interaction of KSHV with endothelial cells. This review will outline novel results and pitfalls of these technologies in the elucidation of KSHV pathogenicity.

Keywords

Endothelial cells - angiogenesis - apoptosis - NF-kappaB - interferon

Full Text

References

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