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Vet Comp Orthop Traumatol 2008; 21(01): 85-88
DOI: 10.3415/VCOT-06-12-0101
Clinical Communication
Schattauer GmbH

[1]1H NMR investigation of normal and osteoarthritic synovial fluid in the horse

L. Lacitignola
1   Dipartimento di Scienze Cliniche Veterinarie, Facoltà di Medicina Veterinaria, Università degli studi di Teramo, Teramo, Italy
,
F. P. Fanizzi
3   Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università di Lecce, Lecce, and Consortium C.A.R.S.O. Cancer Research Center, Bari, Italy
,
E. Francioso
2   D.E.T.O. Veterinary Surgery,University of Bari, Bari, Italy
,
A. Crovace
2   D.E.T.O. Veterinary Surgery,University of Bari, Bari, Italy
› Author Affiliations
Further Information

Publication History

Received 20 December 2006

Accepted 22 March 2007

Publication Date:
17 December 2017 (online)

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Summary

Proton magnetic resonance spectroscopy (1H NMR) has been successfully used in the study of many biological fluids. The data presented here report on the metabolic profiles of normal equine synovial fluids compared with osteoarthritic (OA) fluids. Twenty-five OA synovial fluid samples and eight normal ones were collected from the forelimb fetlock joint in 22 horses, aged between five and 24 years. 1H NMR spectroscopy was carried out with a Bruker Avance DRX 500 equiped with a cryomagnet working at 11 Tesla, and ‘Mestre-C 4.9.9.6’ software was used to analyze the spectra. The study assessed the increase of lactate, alanine, acetate, N-acetylglucosamine, pyruvate, citrate, creatine/creatinine, glycerol, HDL choline, and α-glucose in OA synovial fluid. The variations observed in samples from horses with OA compared to those in the control group, and similar data found in other studies, confirm that this technique may be useful in the study of joint metabolism. Its practical application may be in the evaluation of the treatment of OA in athletic horses.

Keywords

Osteoarthritis - synovial fluid - nucler magnetic resonance spectroscopy - equine
 

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