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Horm Metab Res 1979; 11(2): 85-90
DOI: 10.1055/s-0028-1092685
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© Georg Thieme Verlag KG Stuttgart · New York

Absorption of Protamine-lnsulin in Diabetic Patients - I. Preparation and Characterization of Protamine-125I-InsuIin

B.  Hansen , Susanne  Linde , K.  Kølendorf , Frida  Jensen
  • Research Laboratory, Steno Memorial Hospital and Nordisk Insulinlaboratorium, Gentofte, Denmark
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Publikationsdatum:
17. Dezember 2008 (online)

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Abstract

Protamine-125I-insulin with low specific radioactivity was prepared using 125,127I-insulin, 0.2 I/mole. The preparations were characterized by disc electrophoresis, isoelectric focusing and analytical gel chromatography in order to evaluate the suitability of 125I-insulin as marker for insulin in protamine-insulin. The stability of the preparations was followed up to 90 days at 4°C. The biological and immunological activity was determined in mice, isolated rat fat cells and by radioimmunoassay.

It was concluded that both from a chemical and a biological point of view, the protamine-125I-insulin is a satisfactory preparation that might be used in absorption studies.

Key words

125I-Insulin - Protamine-125I-Insulin - NPH-Iodoinsulin - Absorption of Insulin

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