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Thromb Haemost 1987; 58(04): 1012-1016
DOI: 10.1055/s-0038-1646046
Original Article
Schattauer GmbH Stuttgart

Congenital Deficiency of Alpha-2-Adrenoceptors on Human Platelets : Description of Two Cases

Giacomo Tamponi
*   The Cattedra di Ematologia, Istituto di Medicina Interna, University of Torino, Italy
,
Antonella Pannocchia
*   The Cattedra di Ematologia, Istituto di Medicina Interna, University of Torino, Italy
,
Carlo Arduino
The Istituto di Farmacologia e Terapia Sperimentale, University of Torino, Italy
,
Mario Bazzan
*   The Cattedra di Ematologia, Istituto di Medicina Interna, University of Torino, Italy
,
Nadine Della Dora
*   The Cattedra di Ematologia, Istituto di Medicina Interna, University of Torino, Italy
,
Piercarla Schinco
*   The Cattedra di Ematologia, Istituto di Medicina Interna, University of Torino, Italy
,
Mauro Buraglio
The Istituto di Farmacologia e Terapia Sperimentale, University of Torino, Italy
,
Mario Eandi
The Istituto di Farmacologia e Terapia Sperimentale, University of Torino, Italy
› Author Affiliations
Further Information

Publication History

Received 24 December 1986

Accepted after revision 17 August 1987

Publication Date:
29 June 2018 (online)

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Summary

The biochemistry and functionality of platelets from two related subjects (mother and son) with alpha-2-adrenoceptor-deficient platelets has been evaluated. Radioligand binding experimentes with the specific alpha-2-adrenergic-receptor antagonist, 3H-yohimbine, showed a drastic reduction of alpha-2-adrenoceptors in platelets from both subjects in comparison with the control values. Electron microscopy studies revealed a normal morphology and a normal number of alpha granules and dense bodies. Levels of adenine nucleotides; 5-hydroxytryptamine; B-thromboglobulin; platelet-factor-4 and thromboxane A2 production were within normal limits.

Platelet aggregation and 5-hydroxytryplamine production in response to adrenalin (at concentrations up to 50 μM) were absent, whereas ADP, AA, PAF, collagen and thrombin-induced aggregation, secretion, Ca++ flux and thromboxane A2 production were normal.

The inhibitory effect caused by different concentrations of prostacyclin on Ca++ flux, aggregation, secretion and thromboxane A2 production of platelet functionally lacking of alpha-2-adrenoceptor was not distinguishable from control platelets and platelets preincubated with yohimbine.

Keywords

Alpha-2-adrenoceptors - Human platelets - Congenital deficiency
 

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