Download PDF
Thromb Haemost 1978; 40(02): 518-531
DOI: 10.1055/s-0038-1648685
Original Article
Schattauer GmbH Stuttgart

Platelet Functions in Relation to Dietary Fats in Farmers from two Regions of France[*]

S Renaud
The INSERM, Unité 63, Lyon-Bron, France and the Department of Nutrition, University of Montreal, Montreal, Canada
,
E Dumont
The INSERM, Unité 63, Lyon-Bron, France and the Department of Nutrition, University of Montreal, Montreal, Canada
,
F Godsey
The INSERM, Unité 63, Lyon-Bron, France and the Department of Nutrition, University of Montreal, Montreal, Canada
,
A Suplisson
The INSERM, Unité 63, Lyon-Bron, France and the Department of Nutrition, University of Montreal, Montreal, Canada
,
C Thevenon
The INSERM, Unité 63, Lyon-Bron, France and the Department of Nutrition, University of Montreal, Montreal, Canada
› Author Affiliations
Further Information

Publication History

Received 21 June 1977

Accepted 09 April 1978

Publication Date:
12 July 2018 (online)

  PDF Download  Permissions and Reprints

Summary

To determine whether the long-term feeding of dietary fats affect platelet functions in man, platelet aggregation (to thrombin ADP, collagen, epinephrine) and clotting activity of platelet-rich plasma (PRP), platelet-poor plasma and of washed platelets, were studied in a mobile-laboratory in 44 healthy male farmers (40-45 years) from two French regions Var and Moselle, in relation to lipemia, glycemia, dietary nutriments, and platelet phospholipid composition. In the Moselle subjects, the platelet clotting activity of PRP and of washed platelets, the platelet aggregation to thrombin and ADP, were highly significantly (p <0.001) increased as compared to those of Var, but not the plasma cholesterol, which was identical in the two regions.

In Moselle, the intake of total calories, total lipids and saturated fats was higher than in the Var.

However, it was only with the saturated fat intake (mostly stearic acid) that the platelet clotting activity (p <0.01) and the platelet aggregation (p <0.001) were highly significantly correlated. The platelet clotting activity was also significantly (p <0.001) correlated with the fatty acid composition of the platelet phospholipid fractions phosphatidyl serine + phosphatidyl inositol.

* Presented in part at the IVth International Symposium on Atherosclerosis, August 1976, Tokyo, Japan and at the 50th meeting of the American Heart Association, November 1977, Miami, USA.


 

  • References

  • 1 AOAC(Association of official analytical chemists). 1975 Methods. 12th Edition. Drying upon quartz sand. Official final action 565
    PubMedGoogle Scholar
  • 2 CEE. 1971; Dosage des matières grasses brutes. Journal officiel des Communautés Européennes N° L 279: 17
    PubMedGoogle Scholar
  • 3 CEE. 1972; Dosage des protéines brutes. Journal officiel des Communautés Européennes N° L 123: 9
    PubMedGoogle Scholar
  • 4 Folch J, Lees M, Sloane Stanley GH. 1957; Simple method for the isolation and purification of total lipids from animals tissues. Journal of Biochemistry 226: 497
    PubMedGoogle Scholar
  • 5 Gautheron P, Renaud S. 1972; Hyperlipemia induced hypercoagulable state in rat. Role of an increased activity of platelet phosphatidyl serine in response to certain dietary fatty acids Thrombosis Research 1: 353
    CrossrefPubMedGoogle Scholar
  • 6 Gautheron P, Dumont E, Renaud S. 1974; Clotting activity of platelet phospholipids in rat and man. Thrombosis et Diathesis Haemorrhagica 32: 382
    PubMedGoogle Scholar
  • 7 Hartroft WS. 1958; Abnormal fat transport. Diabetes 7: 221
    CrossrefPubMedGoogle Scholar
  • 8 Hornstra G. 1973; Dietary fats and arterial thrombosis. Haemostasis 2: 21
    PubMedGoogle Scholar
  • 9 Iacono JM, Binder RA, Marshall MW, Schoeme NW, Jencks JA, Mackin JF. 1975; Decreased susceptibility to thrombin and collagen platelet aggregation in man fed a low fat diet. Haemostasis 3: 306
    PubMedGoogle Scholar
  • 10 Inserm. 1972; Statistiques des causes Médicales de Décès. Tome II, Résultats par Région 187: 327
    PubMedGoogle Scholar
  • 11 Keys A. 1970 Coronary heart disease in seven countries. Circulation 41. (Suppl.) 01
    PubMedGoogle Scholar
  • 12 Klatsky AL, Friedman GD, Siegelaub AB. 1974; Alcohol consumption before myocardial consumption. Annals of Internal Medicine 81: 294
    CrossrefPubMedGoogle Scholar
  • 13 Lefevre AF, Decarli LM, Lieber SC. 1972; Effect of ethanol on cholesterol and bile acid metabolism. Journal of lipid Research 13: 48
    PubMedGoogle Scholar
  • 14 Mustard JF, Rowsell HC, Murphy EA, Downie HG. 1963; Diet and thrombus formation: Quantitative studies using an extracorporeal circulation in pigs. Journal of Clinical Investigation 42: 1783
    CrossrefPubMedGoogle Scholar
  • 15 Nichols AB, Ravenscroft C, Lamphicar DE, Ostrander Jr LD. 1976; Daily nutritional intake and serum lipid levels: the Tecumseh study. American Journal of Clinical Nutrition 29: 1384
    CrossrefPubMedGoogle Scholar
  • 16 Nordoy A, Hamlin JT, Chandler AB, Newland H. 1968; The influence of dietary fats on plasma and platelet lipids, and ADP induced platelet thrombosis in the rat. Scandinavian Journal of Haematology 5: 458
    PubMedGoogle Scholar
  • 17 O’Brien JR, Etherington MD, Jamieson S, Vergroesen AJ, Ten Hoor F. Effects of a diet of polyunsaturated fats on some platelet function tests. Lancet 2: 995
    PubMedGoogle Scholar
  • 18 Oliver MF, Nimmo IA, Cooke M, Carlson LA, Olsson AG. 1975; Ischaemic heart disease and associated risk factors in 40 year old men in Edinburgh‘and Stockholm. European Journal of Clinical Investigation 5: 507
    CrossrefPubMedGoogle Scholar
  • 19 Randoin L, Le Gallee P, Dupuis Y, Bernardin A. 1947. Table de composition des Aliments. Institut Scientifique d’Hygiène Alimentaire, publ; 5. edition
    Google Scholar
  • 20 Renaud S. 1969; Thrombotic, atherosclerotic and lipemie effects of dietary fats in the rat. Angiology 20: 657
    CrossrefPubMedGoogle Scholar
  • 21 Renaud S, Kuba K, Goulet C, Lemire Y, Allard C. 1970; a Relationship between fatty acid composition of platelets and platelet aggregation in rat and man. Relation to thrombosis Circulation Research 26: 553
    CrossrefPubMedGoogle Scholar
  • 22 Renaud S, Lecompte F. 1970; b Hypercoagulability induced by hyperlipemia in rat, rabbit and man. Role of platelet factor 3 Circulation Research 27: 1003
    CrossrefPubMedGoogle Scholar
  • 23 Renaud S, Gautheron P. 1973; Dietary fats and experimental(cardiac and venous) thrombosis. Haemostasis 2: 53
    PubMedGoogle Scholar
  • 24 Renaud S, Gautheron P. 1974; a Influence of dietary fats on atherosclerosis, coagulation and platelet phospholipids in rabbit. Atherosclerosis 21: 115
    PubMedGoogle Scholar
  • 25 Renaud S, Gautheron P, Arbogast R, Dumont E. 1974; b Platelet factor 3 activity and platelet aggregation in patients submitted to coronarography. Scandinavian Journal of Haematology 12: 85
    PubMedGoogle Scholar
  • 26 Rosenthal SR. 1934 Studies in atherosclerosis; chemical, experimental and morphologic. Archives of Pathology 18, 473, 600 and 827
    PubMedGoogle Scholar
  • 27 Stamler J. 1973; Epidemiology of coronary heart disease. Medical Clinics of North America 57: 5
    CrossrefPubMedGoogle Scholar
  • 28 Van Handel E, Zilversmit DB. 1957; Micromethod for the direct determination of serum triglycerides. Journal of Laboratory and Clinical Medicine 50: 152
    PubMedGoogle Scholar
  • 29 Watt BK, Merrill AL. 1963. Composition of foods, Agriculture handbook N°8. Agricultural Research Service; United States Department of Agriculture:
    Google Scholar