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Thromb Haemost 1981; 46(02): 515-520
DOI: 10.1055/s-0038-1653400
Original Article
Schattauer GmbH Stuttgart

Platelet Adhesion to Collagen Is Inhibited by Adenosine Diphosphate but Unaffected by Cell Shape

F A Meyer
The Polymer Department, Weizmann Institute of Science, Rehovot, Israel
,
Z Weisman
The Polymer Department, Weizmann Institute of Science, Rehovot, Israel
,
M M Frojmovic
The Polymer Department, Weizmann Institute of Science, Rehovot, Israel
› Author Affiliations
Further Information

Publication History

Received 17 September 1979

Accepted 12 May 1981

Publication Date:
26 July 2018 (online)

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Summary

The effect of limited platelet activation, in the absence of aggregation, on the subsequent ability of rabbit platelets to adhere to collagen was studied in vitro. ADP at concentrations that initiate shape change (>0.01. μM) reduce platelet adhesion. Shape change per se however, was not responsible since the time-dependence of the effect of ADP on shape change and adhesion is different and ADP induces reduced adhesion even when shape change is prevented with PGE1 or fixatives. Platelets shape changed without ADP addition, e. g. by chilling or by low ethanol concentrations, display normal adhesion to collagen

It appears likely that upon binding to the platelet ADP induces a time-dependent alteration in the membrane, akin to refractoriness, that influences binding sites for collagen. The effect of ADP can be blocked by prior addition of AMP but not if the additions are reversed. The implications of the present findings for platelet adhesion studies are discussed.

Keywords

Platelets - Collagen - ADP - Adhesion - Cell shape
 

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