Download PDF
Thromb Haemost 1995; 73(04): 626-629
DOI: 10.1055/s-0038-1653832
Original Articles
Coagulation
Schattauer GmbH Stuttgart

Growth Hormone Deficiency Impairs Blood Clotting and Reduces Factor VII Coagulant Activity in Rat

L S G Sävendahl
1   The Department of Pediatrics, Umeå University Hospital, Umeå, Sweden
,
K Grankvist
2   The Department of Clinical Chemistry, Umeå University Hospital, Umeå, Sweden
,
K G Engström
3   The Department of Histologym and Cell Biology, University of Umeå, Umeå, Sweden
› Author Affiliations
Further Information

Publication History

Received 05 September 1994

Accepted after resubmission 14 December 1994

Publication Date:
26 July 2018 (online)

  PDF Download  Permissions and Reprints

Summary

To investigate pituitary effects on the vitamin K-dependent coagulation factors, female rats were hypophysectomized (hypox) and treated with growth hormone (GH), cortisone, thyroxine, vitamin K, or saline. After 11 days of treatment, the prothrombin time, platelet count, and factors II, VII, IX, and X were determined. The prothrombin time was 52.9 ± 1.2% for control rats and 39.1 ± 0.8% for hypox rats (mean ± SEM; p<0.001). All factors decreased after hypophysectomy, reaching significance for factor VII (from 264 ± 23% to 131 ± 9%; p <0.001) and factor IX (from 28.4 ± 2.2% to 17.1 ± 2.5%; p<0.01) while the platelet count was unaffected. When hypox rats were treated with GH, the prothrombin time increased to 50.9 ± 1.0% (p <0.001) and factor VII to 299 ± 10% (p<0.001). Factor II, IX, and X were slightly increased after GH substitution but not after cortisone, thyroxine, or vitamin K treatment. To summarize, GH is of importance for normal hemostasis in the female rat.

 

  • References

  • 1 Jørgensen JO, Pedersen SA, Ingerslev J, Möler J, Skakkebok NE, Christiansen S. Growth hormone (GH) therapy in GH-deficient patients the plasma factor Vlll-von Willebrand factor complex and capillary fragility. A double-blind, placebo-controlled crossover study Scand J Clin Lab Invest 1990; 50: 417-420
    CrossrefPubMedGoogle Scholar
  • 2 Borkenstein M, Muntean WR. Effects of growth hormone on the factor VIII complex in patients with growth hormone deficiency. Metabolism 1984; 33: 1065-1067
    CrossrefPubMedGoogle Scholar
  • 3 Ingerslev J, Christensen PD, Stenbjerg S, Møller N, Ørskov H, Christiansen JS. Diabetes-like alterations in haemostatic parameters after one week’s growth hormone administration in normal man. J Diabetic Complications 1989; 3: 103-106
    CrossrefPubMedGoogle Scholar
  • 4 Gustafsson J-Å, Edén S, Eneroth P, Hökfelt T, Isaksson O, Jansson J-O, Mode A, Norstedt G. Regulation of sexually dimorphic hepatic steroid metabolism by the somatostatin-growth hormone axis. J Steroid Biochem 1983; 19: 691-698
    CrossrefPubMedGoogle Scholar
  • 5 Eriksson L, Carlsson-Bostedt L, Oscarsson J, Edén S, Stigbrand T, von Schoultz B. Secretory pattern of growth hormone regulates plasma concentration of pregnancy- associated murine protein-1 in the non-pregnant rat. J Reprod Fert 1988; 84: 111-6
    CrossrefPubMedGoogle Scholar
  • 6 Jansson J-O, Oscarsson J, Mode A, Ritzén EM. Plasma growth hormone pattern and androgens influence the levels of corticosteroid-binding globulin in rat serum. J Endocrinol 1989; 122: 725-732
    CrossrefPubMedGoogle Scholar
  • 7 Jeffery S, Carter ND, Clark RG, Robinson IC A F. The episodic secretory pattern of growth hormone regulates liver carbonic anhydrase III. Biochem J 1990; 266: 69-74
    CrossrefPubMedGoogle Scholar
  • 8 Schlechter NL, Russell SM, Greenberg S, Spencer EM, Nicoll CS. A direct growth effect of growth hormone in rat hindlimb shown by arterial infusion. Am J Physiol 1986; 250: E231-E235
    PubMedGoogle Scholar
  • 9 Theeuwes F, Yum SI. Principles of the design and operation of generic osmotic pumps for the delivery of semisolid or liquid drug formulations. Ann Biomed Eng 1976; 4: 343-356
    CrossrefPubMedGoogle Scholar
  • 10 Gordon EM, Hellerstein HK, Ratnoff OD, Arafah BM, Yamashita TS. Augmented Hageman factor and prolactin titers, enhanced cold activation of factor VII, and spontaneous shortening of prothrombin time in survivors of myocardial infarction. J Lab Clin Med 1987; 109: 409-413
    PubMedGoogle Scholar
  • 11 Lundboek K, Christensen NJ, Jensen VA. Diabetes, diabetic angiopathy, and growth hormone. Lancet 1970; 2: 131-133
    PubMedGoogle Scholar
  • 12 Christiansen NJ, Terkilsen AB. Quantitative measurements of skin capillary resistance in hypophysectomized long-term diabetics. Diabetes 1971; 20: 297-301
    CrossrefPubMedGoogle Scholar
  • 13 Edén S. Age- and sex related differences in episodic growth hormone secretion in the rat. Endocrinology 1979; 105: 555-560
    CrossrefPubMedGoogle Scholar
  • 14 Engström KG, Ohlsson L, Oscarsson J. Effect of hypophysectomy and growth hormone substitution on red blood cell morphology and filterability in rats. J Lab Clin Med 1990; 116: 196-205
    PubMedGoogle Scholar
  • 15 Mosher DF. Blood coagulation and fibrinolysis: An overview. Clin Cardiol 1990; 13 (06) 5-11
    CrossrefPubMedGoogle Scholar
  • 16 Porta M, Maneschi F, White MC, Kohner EM. Twenty-five hour variations of von Willebrand factor and factor VIII-related antigen in diabetic retinopathy. Metabolism 1981; 695-699
    PubMedGoogle Scholar
  • 17 Hart A, Cohen H. Capillary fragility studies in diabetes. Brit Med J 1969; 2: 89-91
    PubMedGoogle Scholar
  • 18 Postel-Vinay M-C. Binding of human growth hormone to rat liver membranes: lactogenic and somatotropic sites. FEBS letter 1976; 68: 137-140
    CrossrefPubMedGoogle Scholar
  • 19 Mathews S, Enberg B, Norstedt G. Regulation of rat growth hormone receptor gene expression. J Biol Chem 1989; 264: 9905-9910
    PubMedGoogle Scholar
  • 20 Maiter D, Underwood LE, Maes M, Davenport ML, Ketelslegers JM. Different effects of intermittent and continuous growth hormone (GH) administration on serum somatomedin-C/insulin-like growth factor I and liver GH receptors in hypophysectomized rats. Endocrinology 1988; 123: 1053-1059
    CrossrefPubMedGoogle Scholar
  • 21 Oscarsson J. Growth hormone regulation of serum lipoproteins in the rat. Thesis, University of Göteborg; Sweden: 1990. pp 1-43
    Google Scholar
  • 22 D’Ercole AJ, Stiles AD, Underwood LE. Tissue concentrations of somatomedin C. Further evidence for multiple sites of synthesis and paracrine mechanisms of action. Proc Natl Acad Sci 1984; 81: 935-939
    CrossrefPubMedGoogle Scholar
  • 23 Ekberg S, Carlsson L, Carlsson B, Billig H, Jansson J-O. Plasma growth hormone pattern regulates epidermal growth factor (EGF) receptor messenger ribonucleic acid levels and EGF binding in the rat liver. Endocrinology 1989; 125: 2158-2166
    CrossrefPubMedGoogle Scholar
  • 24 Mann KG, Nesheim ME, Church P, Haley P, Krishnaswamy S. Surface-dependent reactions of the vitamin K-dependent enzyme complexes. Blood 1990; 76: 1-16
    PubMedGoogle Scholar
  • 25 Chemausek SD, Underwood LE, van WykJ. Influence of hypothyroidism on growth hormone binding by rat liver. Endocrinology 1982; 111: 1534-1538
    CrossrefPubMedGoogle Scholar
  • 26 Primrose JN, Davies JA, Prentice CR M, Hughes R, Johnston D. Reduction in factor VII, fibrinogen and plasminogen activator inhibitor-1 activity after surgical treatment of morbid obesity. Thromb Haemost 68: 396-399
    Article in Thieme ConnectPubMedGoogle Scholar
  • 27 Folsom AR, Qamhieh HT, Wing RR, Jeffery RW, Stinson VL, Kuller LH, Wu KK. Impact of weight loss and plasminogen activator inhibitor (PAI-I), factor VII, and other hemostatic factors in moderately overweight adults. Arteriosclerosis 1993; 13: 162-169
    CrossrefPubMedGoogle Scholar
  • 28 Miller G J. Hemostasis and cardiovascular risk. The British and European experience Arch Pathol Lab Med 1992; 116: 1318-1321
    PubMedGoogle Scholar
  • 29 Scarabin PY, Bonithon-Kopp C, Bara L, Malmejac A, Guize L, Samama M. Factor VII activation and menopausal status. Thromb Res 1990; 57: 227-234
    CrossrefPubMedGoogle Scholar
  • 30 Schulman S, Johnsson H, Lindmarker P. Thrombotic complications after substitution with a FVII concentrate. Thromb Haemost 1991; 66: 619
    Article in Thieme ConnectPubMedGoogle Scholar
  • 31 Bengtsson BÅ, Edén S, Ernest I. Epidemiology and long-term survival in acromegaly A study of 166 cases diagnosed between 1955 and 1984. Acta Med Scand 1988; 223: 327-35
    PubMedGoogle Scholar
  • 32 Wright AD, Hill DM, Lowy C. Mortality in acromegaly. Q J Med 1970; 39: 1-16
    PubMedGoogle Scholar