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Plant Biol (Stuttg) 2004; 6(1): 91-99
DOI: 10.1055/s-2003-44718
Original Paper

Georg Thieme Verlag Stuttgart · New York

Compatible and Incompetent Paxillus involutus Isolates for Ectomycorrhiza Formation in vitro with Poplar (Populus × canescens) Differ in H2O2 Production

A. Gafur 1 , A. Schützendübel 2 , R. Langenfeld-Heyser 2 , E. Fritz 2 , A. Polle 2
  • 1Department of Plant Protection, University of Lampung, Bandar Lampung, Indonesia
  • 2Forstbotanisches Institut, Georg-August University Göttingen, Göttingen, Germany
Further Information

Publication History

Publication Date:
17 February 2004 (online)

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Abstract

Isolates of Paxillus involutus (Batsch) Fr. collected from different hosts and environmental conditions were screened for their ability to form ectomycorrhizal symbiosis with hybrid poplar P. × canescens (= Populus tremula L. × P. alba) in vitro. The ability to form ectomycorrhiza varied between the fungal isolates and was not correlated with the growth rate of the fungi on agar-based medium. The isolate MAJ, which was capable of mycorrhiza synthesis under axenic conditions, and the incompetent isolate NAU were characterized morphologically and anatomically. MAJ formed a typical hyphal mantle and a Hartig net, whereas NAU was not able to penetrate the host cell walls and caused thickenings of the outer cell walls of the host. MAJ, but not NAU, displayed strong H2O2 accumulation in the outer hyphal mantle. Increases in H2O2 in the outer epidermal walls and adjacent hyphae of the incompetent isolate were moderate. No increases of H2O2 in response to the mycobionts were found inside roots. Suggested functions of H2O2 production in the outer hyphal mantle of the compatible interaction are: growth regulation of the host's roots, defence against other invading microbes, or increasing plant-innate immunity. The system established here for P. × canescens compatible and incompetent fungal associations will be useful to take advantage of genomic information now available for poplar to study tree-fungal interactions at the molecular and physiological level.

Key words

Defence signalling - mycorrhiza - oxidative stress - Paxillus involutus - poplar - symbiosis.

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A. Polle

Forstbotanisches Institut
Georg-August-Universität Göttingen

Büsgenweg 2

37077 Göttingen

Germany

Email: apolle@gwdg.de

Section Editor: H. Rennenberg

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