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Horm Metab Res 1983; 15(10): 485-487
DOI: 10.1055/s-2007-1018765
© Georg Thieme Verlag, Stuttgart · New York

Immunocytochemical Localization of the Glycoprotein Hormone Alpha Subunit in the Median Eminence of the Mouse Hypothalamus

Nina L. Schorr-Toshav, N. S. Halmi1 , J. M. Wurzel, Ione A. Kourides
  • Laboratory of Molecular Endocrinology, Memorial Sloan-Kettering Cancer Center and Cornell University Medical College, New York, U.S.A.
  • 1Department of Anatomy, Mount Sinai School of Medicine, New York, U.S.A.
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Publication History

1982

1982

Publication Date:
14 March 2008 (online)

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Summary

Immunoperoxidase staining was performed to localize glycoprotein hormone alpha subunit in mouse brain. Staining was found only in the median eminence with the use of ovine LH-alpha antiserum and was diffuse, not localized to fibers but to pericapillary areas. Absorption of the antiserum with bovine TSH-alpha subunit completely abolished the staining. These findings, in conjunction with our earlier studies in which alpha subunit messenger RNA was not detected in mouse brain, suggest that alpha subunit reaches the hypothalamus by retrograde diffusion from the pituitary.

Key-Words:

Immunocytochemistry - Alpha Subunit of Glycoprotein Hormones - Mouse Brain

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