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Int J Sports Med 2007; 28(8): 650-654
DOI: 10.1055/s-2007-964855
Physiology & Biochemistry

© Georg Thieme Verlag KG Stuttgart · New York

The Effect of Basketball Training on the Players' Erythrocyte Membrane Acetylcholinesterase, (Na+,K+)-ATPase and Mg2+-ATPase Activities

T. Parthimos1 , C. Tsopanakis1 , P. Angelogianni1 , K. H. Schulpis2 , N. Parthimos1 , S. Tsakiris1
  • 1Department of Experimental Physiology, Medical School, University of Athens, Athens, Greece
  • 2Department of Metabolic Disorders, Institute of Child Health, “Aghia Sophia” Children's Hospital, Athens, Greece
Further Information

Publication History

accepted after revision July 6, 2006

Publication Date:
13 April 2007 (online)

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Abstract

The aim of this study was to investigate whether the activities of erythrocyte membrane acetylcholinesterase (AChE), (Na+,K+)-ATPase and Mg2+-ATPase are modulated by a basketball training. Blood was obtained from 10 basketball players pre- and postexercise. Total antioxidant status (TAS), lactate and pyruvate concentrations were determined with kits, while the enzyme activities were determined spectrophotometrically. Post-training blood lactate and pyruvate concentrations as well as AChE (2.90 ± 0.05 vs. 3.98 ± 0.09 ΔOD/min · mg protein, p < 0.01) and Na+,K+-ATPase (0.58 ± 0.04 vs. 1.27 ± 0.12 µmol Pi/h · mg protein, p < 0.001) activities were remarkably increased, whereas TAS was significantly decreased. Mg2+-ATPase activity remained unaltered at the end of the training. In conclusion, the stimulation of AChE and Na+,K+-ATPase by the training may be due to the rise of blood catecholamine oxidation contributing to TAS decrease and/or the increase of serotonin levels. This stress condition may modulate cholinergic and catecholaminergic/serotoninergic functions in players.

Key words

exercise - oxidative stress - ATPase - acetylcholinesterase - erythrocyte membranes - lactate

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Prof. Stylianos Tsakiris

Department of Experimental Physiology
Medical School
University of Athens

PO Box 65257

15401 Athens

Greece

Email: stsakir@cc.uoa.gr

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