Horm Metab Res 1980; 12(12): 660-664
DOI: 10.1055/s-2007-999227
© Georg Thieme Verlag, Stuttgart · New York

Immunoreactive Dog C-Peptide Level in the Pancreatic Vein

K. Kawanishi, Y. Nishina, T. Ishida, S. Machida, S. Yamamoto, T. Ofuji, N. Sakura1 , N. Yanaihara1
  • Third Department of Internal Medicine, Okayama University Medical School, Okayama, Japan
  • 1Laboratory of Bioorganic Chemistry, Shizuoka College of Pharmacy, Shizuoka, Japan
Further Information

Publication History

1978

1980

Publication Date:
22 April 2008 (online)

Summary

C-peptide immunoreactivity (CPR) levels were measured in dog superior pancreaticoduodenal vein using synthetic dog C-peptide and its antiserum. The basal CPR level was approximately twice as high as the basal immunoreactive insulin (IRI) level on a molar basis. Glucose (10 mg/kg/min) or arginine (250 mg/kg/min) infusion for 5 min into the superior pancreaticoduodenal artery caused a prompt, parallel increase in IRI and CPR. IRI and CPR were closely equimolar at peak secretions. One bolus administration of synthetic neurotensin (10 μg/kg) into the same artery produced a mild hyperglycemic response and biphasic IRI and CPR responses at 30 min in the vein. The IRI and CPR increases were closely equimolar during the first phase of secretion, but during the second peak a larger increase was found in CPR than IRI. Upon infusion of synthetic substance P (50 ng/kg/min) for 30 min, IRI and CPR concentrations showed a parallel and closely equimolar fall. These results indicate that insulin and C-peptide were released from beta cells in equimolar concentrations.