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Horm Metab Res 2008; 40(7): 454-458
DOI: 10.1055/s-2008-1065323
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Type 1 Diabetes Alters Brain Cannabinoid Receptor Expression and Phosphorylation Status in Rats

C. Díaz-Asensio 1 , R. Setién 1 , E. Echevarría 1 , L. Casis 1 , E. Casis 2 , A. Garrido 2 , O. Casis 1
  • 1Department of Physiology, School of Pharmacy, University of the Basque Country, Bilbao, Spain
  • 2Clinical Chemistry Laboratory, Hospital Donostia, San Sebastián, Spain
Further Information

Publication History

received 01.06.2007

accepted 04.12.2007

Publication Date:
27 March 2008 (online)

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Abstract

One of the most common symptoms of diabetes is extreme hunger, but the brain mechanism underlying this hyperphagia is unknown. The endocannabinoid system has emerged as one of the main food intake regulators in the brain. However, the effects of type 1 diabetes on the endocannabinoid system are not completely known. Thus, the aim of the present work is to establish the possible alterations induced by type 1 diabetes on the brain endocannabinoid system in rats. Western blot and immunocytochemistry were used to measure CB1 and phosphorylated CB1 receptor expression in several prosencephalic regions in streptozotocin-induced type 1 diabetic rats. Serum leptin levels were measured by ELISA. CB1 receptor expression was increased in striatum and hypothalamus of diabetic animals, with no changes in other brain areas studied. CB1 receptor phosphorylation was also increased in the same brain areas. Type 1 diabetes induced significant weight loss, and serum leptin levels were severely decreased. These results reinforce the possible role of the CB1 receptor as a pharmacological target for the clinical management of appetite in diabetic patients.

Key words

CB1 - hyperphagia - appetite - insulin - weight loss

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Correspondence

Dr. O. Casis

Departamento de Fisiología

Universidad del País Vasco

P.O. Box 699

48080 Bilbao

Spain

Phone: +34/94/601 28 44

Fax: +34/94/601 56 62

Email: oscar.casis@ehu.es