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Thromb Haemost 2007; 97(01): 11-14
DOI: 10.1160/TH06-09-0524
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Alternatively spliced human tissue factor (asHTF) is not pro-coagulant

Petra Censarek   *
1   Institut für Pharmakologie und Klinische Pharmakologie, Universitätsklinikum Düsseldorf, Germany
,
Anett Bobbe   *
1   Institut für Pharmakologie und Klinische Pharmakologie, Universitätsklinikum Düsseldorf, Germany
,
Maria Grandoch
2   Institut für Pharmakologie, Universität Duisburg-Essen, Universitätsklinikum, Germany
,
Karsten Schrör
1   Institut für Pharmakologie und Klinische Pharmakologie, Universitätsklinikum Düsseldorf, Germany
,
Artur-Aron Weber
1   Institut für Pharmakologie und Klinische Pharmakologie, Universitätsklinikum Düsseldorf, Germany
› Author Affiliations
Further Information

Publication History

Received 15 September 2006

Accepted after revision 14 November 2006

Publication Date:
30 November 2017 (online)

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Summary

It has been proposed that alternatively-spliced human tissue factor (asHTF) is pro-coagulant. We have evaluated the function of asHTF in a mammalian expression system. Full-length human tissue factor (HTF) and asHTF were cloned from smooth muscle cells and over-expressed in HEK293 cells. As expected, a marked pro-coagulant activity (FX activation, thrombin generation) was observed on the surface, in lysates, and on microparticles from HTF transfected cells. In contrast, no pro-coagulant activity of as HTF was observed.

Keywords

Coagulation factors - tissue factor / factor VII - thrombosis

* These authors contributed equally.


 

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