Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596251
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

LC-DAD-ESI-MS/MS, total phenolic content, and antioxidant activity of Firmiana simplex

Authors

  • MA Ghareeb

    1   Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Kornish El-Nile, 12661 Warrak El-Hadar, Giza, Egypt

  • T Mohamed

    1   Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Kornish El-Nile, 12661 Warrak El-Hadar, Giza, Egypt
    2   Institute of Pharmacy and Molecular Biotechnology, Department of Pharmaceutical Biology, Heidelberg University, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany

  • AM Saad

    1   Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Kornish El-Nile, 12661 Warrak El-Hadar, Giza, Egypt

  • LA Refahy

    1   Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Kornish El-Nile, 12661 Warrak El-Hadar, Giza, Egypt

  • MA Sobeh

    2   Institute of Pharmacy and Molecular Biotechnology, Department of Pharmaceutical Biology, Heidelberg University, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany

  • M Wink

    2   Institute of Pharmacy and Molecular Biotechnology, Department of Pharmaceutical Biology, Heidelberg University, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany
Further Information

Publication History

Publication Date:
14 December 2016 (online)

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The total phenolic content and the antioxidant activity of different extracts from Firmiana simplex (L.) fruits were investigated using Folin-Ciocalteu's reagent, 2,2'-diphenyl-1-picrylhydrazyl radical (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and ferric reducing antioxidant power (FRAP) assays [1 – 3]. LC-DAD-ESI-MS/MS (positive and negative ionization) was used to identify the chemical composition of the most active extract [4]. Ethyl acetate (EtOAc) fraction demonstrated the highest phenolic content with 314.61, followed by 297.51, 153.75, 101.47, 97.19 for dichloromethane (DCM), butanol (BuOH), methanol (MeOH) and water (H2O), respectively, while the lowest value of 27.43 (mg GAE/g dry extract) was obtained for the petroleum ether fraction. The antioxidant activity of extracts correlated with their total phenolic content. IC50 values of the EtOAc, DCM, BuOH, MeOH, H2O and petroleum ether were 6.79, 19.92, 24.78, 28.87, 49.03 and 95.21 µg/ml, respectively, for the DPPH assay, compared to 2.92 of the positive standard ascorbic acid, whereas those of the ABTS assay were 2.11, 3.93, 6.38, 6.68, 13.27 and 40.84 µg/ml, respectively, compared to 1.63 of the positive standard trolox. IC50 values of the FRAP assay were 32.1, 26.62, 17.7, 13.35, 8.24 and 4.12 mM FeSO4 equivalent/mg dry extract, compared to 21.45 of the positive standard quercetin. LC-DAD-ESI-MS/MS of the EtOAc extract revealed the presence of phenolic acids (benzoic acid and cinnamic acid derivatives), flavonoids and tannins. This is the first phytochemical profiling of Firmiana simplex by LC-DAD-ESI-MS/MS.

Keywords: Firmiana simplex (L.), LC-DAD-ESI-MS/MS, TPC, DPPH, FRAP, ABTS.

References:

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No conflict of interest has been declared by the author(s).