Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596648
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Marine halogenated compound analysis: from an R package to the isolation of new griseophenone derivatives

Authors

  • C Roullier

    1   Groupe Mer, Molécules, Santé-EA 2160, UFR des Sciences Pharmaceutiques et Biologiques, Université de Nantes, 9 Rue bias, 44000 Nantes, France
    2   Biogenouest, Corsaire-Thalassomics, Nantes, France

  • Y Guitton

    1   Groupe Mer, Molécules, Santé-EA 2160, UFR des Sciences Pharmaceutiques et Biologiques, Université de Nantes, 9 Rue bias, 44000 Nantes, France
    3   LUNAM Université, Oniris, Laboratoire d'Étude des Résidus et Contaminants dans les Aliments (LABERCA), 44307 Nantes, France

  • S Prado

    4   Molécules de Communication et Adaptation des Micro-organismes, UMR 7245 CNRS/MNHN, Muséum National d'Histoire Naturelle, 75231 Paris Cedex 05, France

  • O Grovel

    1   Groupe Mer, Molécules, Santé-EA 2160, UFR des Sciences Pharmaceutiques et Biologiques, Université de Nantes, 9 Rue bias, 44000 Nantes, France
    2   Biogenouest, Corsaire-Thalassomics, Nantes, France

  • YF Pouchus

    1   Groupe Mer, Molécules, Santé-EA 2160, UFR des Sciences Pharmaceutiques et Biologiques, Université de Nantes, 9 Rue bias, 44000 Nantes, France
Further Information

Publication History

Publication Date:
14 December 2016 (online)

Also available at
 
 

A collection of culture extracts obtained from several marine-derived fungal strains collected on the French Atlantic coast was investigated by HPLC-HRMS in order to prospect for halogenated compounds and to identify potentially original ones. To achieve a fast, automated and efficient data analysis, a bioinformatics tool named MeHaloCoA (Marine Halogenated Compound Analysis) was developed and included into R as a package. After extraction from the metabolic fingerprints of all the peaks and their associated mass spectra, a mathematical filter based on mass isotopic profiles allowed the selective detection of halogenated molecules. Integrating MeHaloCoA into a dereplication approach allowed the identification of known and new halogenated compounds in a challenging amount of time. Subsequent targeted purification led to the isolation of several halogenated metabolites including two new natural products with bioactive potential.

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Acknowledgements: The authors acknowledge the Region des Pays de la Loire for funding of this study through the CHIMIMAR program and the technical platform Corsaire-ThalassOMICS of Biogenouest.

Keywords: Halogenated Natural Products, dereplication, Mass Spectrometry, automated method.


No conflict of interest has been declared by the author(s).

 
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