Planta Med 2019; 85(18): 1441
DOI: 10.1055/s-0039-3399774
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

Quantitative metabolomics of Iranian saffron based on their HPLC-DAD and MALDI-TOF-MS

M Hooshyari
1   Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University,, Tehran, Iran
,
H Rezadoost
1   Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University,, Tehran, Iran
,
P Ghezellou
1   Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University,, Tehran, Iran
,
A Ghassempour
1   Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University,, Tehran, Iran
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

 
 

More than 85% of saffron (Crocus sativus L.) is producing in 22 region of Iran [1]. The quality control and its geographical effects is the main concern behind it. In this study, we developed a chromatographic fingerprint of 22 type saffron from 22 regions of Iran. Multivariate clustering methods of PCA and HCA are used for finding similarities and dissimilarities in three wavelengths 440, 308, 254 lead to the samples divided in different class base on their HPLC fingerprints. Then as a reliable, sensitive and fast method of analysis, MALDI-TOF-MS introduced for simultaneous determination of crocins, safranal and picrocrocin. Eventually and according to International Committee of Harmonization (ICH) regulation for the validation of analytical methods parameters including linearity range for crocins, picrocrocin and safranal was obtained at the range of 22-200, 32-200 and 22-100 ng/spot respectively. Also limit of quantification (LOQ) for crocins, picrocrocin and safranal were 7.18, 10.71 and 6.76 ng/spot respectively. Finally, this work is introducing MADLI-TOF as a very fast and reliable method for quantitative metabolites profiling of medicinal plant.


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  • References

  • 1 Kabiri M, Rezadoost H, Ghassempour A. A comparative quality study of saffron constituents through HPLC and HPTLC methods followed by isolation of crocins and picrocrocin. LWT Food Sci Technol 2017; 84: 1-9

  • References

  • 1 Kabiri M, Rezadoost H, Ghassempour A. A comparative quality study of saffron constituents through HPLC and HPTLC methods followed by isolation of crocins and picrocrocin. LWT Food Sci Technol 2017; 84: 1-9