Planta Med 2019; 85(18): 1522
DOI: 10.1055/s-0039-3400015
Main Congress Poster
Poster Session 2
© Georg Thieme Verlag KG Stuttgart · New York

Chemical composition, direct and indirect antibacterial activity against multidrug resistant bacteria and toxicity assessment of essential oil of Cymbopogon giganteus leaves

H Toukourou
1   Laboratoire de Chimie Pharmaceutique Organique, Ecole de Pharmacie, Faculté des Sciences de la Santé, Université d’Abomey-Calavi,, Campus du champ de Foire, 01BP 188, Cotonou, Bénin
2   Pharmacognosy Research Group, Louvain Drug Research Institute, Université catholique de Louvain,, B1 7203 Av. E. Mounier 72, B-1200 Bruxelles, Belgium
,
H Sounouvou
1   Laboratoire de Chimie Pharmaceutique Organique, Ecole de Pharmacie, Faculté des Sciences de la Santé, Université d’Abomey-Calavi,, Campus du champ de Foire, 01BP 188, Cotonou, Bénin
2   Pharmacognosy Research Group, Louvain Drug Research Institute, Université catholique de Louvain,, B1 7203 Av. E. Mounier 72, B-1200 Bruxelles, Belgium
,
L Catteau
2   Pharmacognosy Research Group, Louvain Drug Research Institute, Université catholique de Louvain,, B1 7203 Av. E. Mounier 72, B-1200 Bruxelles, Belgium
,
F Toukourou
3   Laboratoire de Microbiologie et des Technologies Alimentaires, Champ de Foire ISBA, Faculté des Sciences et Techniques, Université d’Abomey-Calavi,, BP 526 Cotonou, Benin
,
F Van Bambeke
4   Pharmacologie cellulaire et moléculaire, Louvain Drug Research Institute, Université catholique de Louvain,, Avenue Mounier 73, B1.73.05, 1200 Brussels, Belgium
,
F Gbaguidi
1   Laboratoire de Chimie Pharmaceutique Organique, Ecole de Pharmacie, Faculté des Sciences de la Santé, Université d’Abomey-Calavi,, Campus du champ de Foire, 01BP 188, Cotonou, Bénin
,
J Quetin-Leclercq
2   Pharmacognosy Research Group, Louvain Drug Research Institute, Université catholique de Louvain,, B1 7203 Av. E. Mounier 72, B-1200 Bruxelles, Belgium
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Publikationsverlauf

Publikationsdatum:
20. Dezember 2019 (online)

 
 

Because of antibioresistance rising [1], the search of new alternative strategies by combining classic antibiotics and essentials oils to restore antibiotics efficacy may be a promising approach [2]. The aim of this work was to study the direct and indirect antimicrobial activity of Cymbopogon giganteus essential oil from Benin (EOCG) on multidrug resistant bacteria, its chemical composition and its oral acute toxicity. Direct antimicrobial activity was tested by determination of Minimal Inhibitory Concentration (MIC), and indirect activity, by calculating the Fractional Inhibitory Concentration Index using checkerboard (FICI; synergy: FICI ≤ 0.5; additivity: 0.5<FICI≤1) on reference but also on multidrug resistant clinical isolates. Composition was determined by GC-MS and GC-FID. Cytotoxicity was evaluated in vitro against human non-cancer fibroblast cell line (WI38) by MTT assay and oral acute toxicity by determination of the “limit dose test” at 2000mg/kg [3]. Limonene (12.07%) and p-menthane derivatives (54.87%) were the major components. Our results confirmed the direct antimicrobial activity of EOCG, but here on clinical resistant strains (MIC from 0.125%v/v to 0.5%v/v). We also observed, for the first time, the synergistic effects between EOCG and amoxicillin with FICI between 0.12-0.5 against two Escherichia coli amoxicillin-resistant clinical strains, synergistic to additive effects between EOCG and colistin or oxacillin/ampicillin respectively against Pseudomonas aeruginosa PA544 and Staphylococcus epidermidis SE361 (two multiresistant clinical isolates). EOCG had a low cytotoxicity (IC50: 67.06±2.69 μg/ml) and no acute toxicity at the dose of 2000mg/kg per os. This is the first report of oral acute toxicity assessment of this essential oil.


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  • References

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  • References

  • 1 WHO. Antimicrobial resistance: global report on surveillance. World Health Organization; 2014
    Google Scholar
  • 2 Langeveld WT, Veldhuizen EJ, Burt SA. Synergy between essential oil components and antibiotics:a review. Crit Rev Microbiol 2014; 40: 76-94
    CrossrefPubMedGoogle Scholar
  • 3 OECD. OECD Guideline for testing chemicals. Acute oral toxicity-acute toxic class method, guideline no. 423. adopted 2001 Organisation for Economic and Cooperation Development. 2001
    PubMedGoogle Scholar