GC-MS, HPLC analysis and antioxidant estimation of n-hexane and methanol leaf extracts of Spondias mombin growing in Bayelsa State, Nigeria

Medicinal plants are considered as a rich source of phytocompounds which have therapeutic values and so can be used in drug development and synthesis [1]. Natural antioxidants such as flavonoids have shown to play a key role in protecting human body from the damaging effects of free radicals [2]. The leaves of Spondias mombin are commonly used in Bayelsa State, Nigeria for ethnomedicinal purposes to treat diabetes, diarrhea, dysentery, cold, and gonorrhea. This study was carried out to evaluate the antioxidants properties and phytochemical composition of n-hexane and methanol leaf extracts of Spondias mombin plant using GC-MS and HPLC. The antioxidants evaluation of the plant extracts showed that the total phenol, total flavonoid content and total antioxidant capacity were found to be in the range of 9.99 ± 2.26 (mgGAE/g), 9.99 – 23.40 ± 0. 11 (mgQE/g) and 7.00 – 10.02 ± 5.04 (mgAAE/g) dry extract respectively. GC-MS analysis showed the presence of many bioactive components which include longifolene, stigmasterol ester, retinoic acid methyl ester, phytol, ergost-25-ene − 3,5,6,12-tetrol phthalic acid ester, octadecadienoic acid methyl eater, limonene pivalate, n-tetracosanol and bisnorhopane. Also, the HPLC analysis of the methanol extract ([Fig. 1]) gave the following major flavonoids – (+) catechin, apigenin, naringenin, luteolin, kaemferol, quercetin, isorhamnetin and myricetin. The present study provides evidence that the leaf extracts of Spondias mombin are a potential source of natural antioxidants which may act as a chemo-preventative agent.

Fig. 1 

No conflict of interest.

• References

• 1 Rasool Hassan BA. Medicinal plants (importance and uses). Pharmaceut Anal Acta 2012; 3 (10) 2153-2435
• 2 Amos-Tautua B, Ajileye O, Ndoni S. et al. Evaluation of phenolic contents, free radical scavenging activity and functional group analysis of the leaf extract of a medicinal plant in Niger Delta Region. Chem Int 2017; 3: 250

Publikationsverlauf

Artikel online veröffentlicht:
12. Dezember 2022