CC BY-NC-ND 4.0 · Planta Medica International Open 2021; 8(01): e34-e42
DOI: 10.1055/a-1375-6456
Original Article

Extraction and Fractionation Effects on Antiplasmodial Activity and Phytochemical Composition of Palicourea hoffmannseggiana

Leticia Hiromi Ohashi
1  Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Pará, Guamá, Belém, PA, Brazil
2  Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Pampulha, Belo Horizonte, MG, Brazil
,
Douglas Costa Gontijo
2  Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Pampulha, Belo Horizonte, MG, Brazil
,
Maria Fernanda Alves do Nascimento
2  Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Pampulha, Belo Horizonte, MG, Brazil
,
Luciano Ferreira Margalho
3  Instituto Federal do Pará, Icuí-Guajará, Ananindeua, PA, Brazil
,
Geraldo Célio Brandão
4  Escola de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil
,
Alaíde Braga de Oliveira
1  Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Pará, Guamá, Belém, PA, Brazil
2  Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Pampulha, Belo Horizonte, MG, Brazil
› Author Affiliations

Abstract

The present study on Palicourea hoffmannseggiana, which was collected at Marapanim, state of Pará, Brazil, comprises the preparation of different stem and leaf extracts and fractions. Ethanol, hydroethanol, and water extracts were prepared by several methods and evaluated for in vitro activity against resistant Plasmodium falciparum (W2 strain), disclosing a low parasite growth inhibition effect (< 50%). Dereplication by UPLC-DAD-ESIMS of the leaf ethanol extract showed the presence of two known alkaloids, lyalosidic and strictosidinic acids, along with a sinapoyl ester of lyalosidic acid, with m/z 719.33 [M+H]+, which is possibly a new monoterpene indole alkaloid representative. Sequential liquid-liquid acid-base alkaloid separations from the leaf ethanol extract as well as directly from leaf powder afforded fractions of increased parasite growth inhibition, reaching up to 92.5±0.7%. The most bioactive fractions were shown to contain the β-carboline alkaloids harmane and 4-methyl-β-carboline, along with N-methyl-tryptamine and N-acetyl-tryptamine, while monoterpene indole alkaloids were detected in inactive fractions of these processes. The present results demonstrate that these preliminary fractionation methods can lead to significantly active fractions supporting an adequate scale-up to carrying out the isolation of anti-plasmodial compounds.

Supplementary Material



Publication History

Received: 18 October 2020
Received: 19 January 2021

Accepted: 25 January 2021

Publication Date:
19 April 2021 (online)

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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