Klin Monbl Augenheilkd 2024; 241(04): 562-570
DOI: 10.1055/a-2264-5559
Experimentelle Studie

Imaging Assessment of Peripapillary Vessel Diameters in Postmortem Eyes

Bildaufnahmengestützte Erhebung peripapillärer Gefäßdurchmesser in Post-mortem-Augen
Simona Schütz
1   Department of Ophthalmology, University Hospital of Zurich, Switzerland
,
1   Department of Ophthalmology, University Hospital of Zurich, Switzerland
,
1   Department of Ophthalmology, University Hospital of Zurich, Switzerland
2   Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden
,
1   Department of Ophthalmology, University Hospital of Zurich, Switzerland
,
Isabelle Meneau
1   Department of Ophthalmology, University Hospital of Zurich, Switzerland
,
Frank Blaser
1   Department of Ophthalmology, University Hospital of Zurich, Switzerland
,
Mario Damiano Toro
3   Eye Clinic, Department of Public Health, University of Naples Federico II, Napoli, Italy
4   Chair and Department of General and Paediatric Ophthalmology, Medical University of Lublin, Poland
,
Magdalena Rejdak
1   Department of Ophthalmology, University Hospital of Zurich, Switzerland
,
1   Department of Ophthalmology, University Hospital of Zurich, Switzerland
,
1   Department of Ophthalmology, University Hospital of Zurich, Switzerland
› Author Affiliations
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Abstract

Purpose Proof of concept of ex vivo retinal vessel diameter measurements in human postmortem eyes.

Methods En face near-infrared (IR) images and optical coherence tomography (OCT) of the optic nerve head (ONH) were captured ex vivo with a Heidelberg Engineering Spectralis (Spectralis, version 7.0.4, Image Capture Module, version 1.2.4, Heidelberg Heidelberg, Germany) device, using a custom-made eye chamber holding and positioning the eyes during the image process. Thirty-two formaldehyde-fixated eyes of 16 patients were imaged. In the IR images, two independent graders measured retinal vessel diameters at the intersection of a drawn circle centered on the ONH with diameters of 2.0 mm and 3.4 mm, respectively. The anatomically corresponding measurements between both graders were statistically analyzed using a Wilcoxon signed-rank test.

Results A total of 246 matched measurements of both graders were analyzed across all 32 imaged eyes. Statistically significant differences between the graders were found for arterioles at 2 mm from the ONH. The other measurements did not show statistically significant intergrader differences. The mean values for arteriole diameters were 72.2 µm at 2.0 mm and 61.5 µm at 3.4 mm for grader 1, and 66.4 µm at 2.0 mm and 63.2 µm at 3.4 mm for grader 2. The mean diameter for venules were 75.5 µm at 2.0 mm and 79.3 µm at 3.4 mm for grader 1, and 67.4 µm at 2 mm and 79.1 µm at 3.4 mm for grader 2.

Conclusion To the best of our knowledge, this is the first study to present IR image-based retinal vessel diameters in ex vivo postmortem eyes. Retinal IR/OCT imaging is possible, and measurements are reproducible in formaldehyde-fixated human eyes. Fixation artefacts result in lower image quality, and this can impose challenges in correctly detecting, classifying, and measuring retinal vessels.

Zusammenfassung

Messung retinaler Gefäßdurchmesser in menschlichen postmortalen Augen durch zwei unabhängige Personen. Die Studie zeigt, dass die ex vivo IR-/OCT-Bildgebung der Netzhaut möglich ist und Messungen an formaldehydfixierten Augen reproduzierbar sind, wobei Fixierungsartefakte eine Herausforderungen bei der korrekten Erkennung, Klassifizierung und Messung retinaler Gefässe darstellen können.

Supporting Information



Publication History

Received: 29 October 2023

Accepted: 05 February 2024

Article published online:
23 April 2024

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