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Synfacts 2010(9): 1014-1014  
DOI: 10.1055/s-0030-1257922
Synthesis of Materials and Unnatural Products
© Georg Thieme Verlag Stuttgart ˙ New York

A New Animal at the Fluorescence Zoo

Contributor(s): Timothy M. Swager, Olesya Haze
V. N. Belov*, C. A. Wurm*, V. P. Boyarskiy, S. Jakobs, S. W. Hell*
Max Planck Institute for Biophysical Chemistry, Göttingen, Germany
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Publication History

Publication Date:
23 August 2010 (online)

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Significance

Photo-Wolff rearrangement is used to uncage masked fluorescent dyes (1). The new α-diazoketone cage is easily incorporated into rhodamine derivatives. Incorporation of an ester enables bioconjugation and the dyes perform well in intracellular imaging. Reported α-diazoketone caged dyes exhibit a shoulder extending to 420 nm in their absorption spectrum and can be ­photoactivated both at 360-375 nm and longer (405-420 nm) wavelengths. This allows for flexibility in designing single detector multidye imaging schemes.