Synlett 2014; 25(10): 1345-1349
DOI: 10.1055/s-0033-1341024
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© Georg Thieme Verlag Stuttgart · New York

Upgrading Nature’s Tools: Expression Enhancement and Preparative Utility of the Halogenase RebH

James T. Payne
Department of Chemistry, University of Chicago, Chicago, IL 60637, USA   Fax: +1(773)7020805   Email: jaredlewis@uchicago.edu
,
Jared C. Lewis*
Department of Chemistry, University of Chicago, Chicago, IL 60637, USA   Fax: +1(773)7020805   Email: jaredlewis@uchicago.edu
› Author Affiliations
Further Information

Publication History

Received: 07 December 2013

Accepted after revision: 25 February 2014

Publication Date:
03 April 2014 (online)

Abstract

Enzymes often excel at performing green, selective chemical reactions, and thus offer key advantages over many chemocatalytic processes. We recently reported the enhanced expression of a regioselective halogenase, RebH, in E. coli, as well as its use in the preparative halogenation of a range of substrates. These improvements have made it possible to begin directed evolution to improve further the activity and substrate scope of RebH. Chemistry is in need of a regioselective halogenation catalyst, and this work illustrates the potential and recent gains made towards using enzymes for this goal.

 
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